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Journal of Clinical Microbiology, June 2005, p. 2559-2562, Vol. 43, No. 6
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.6.2559-2562.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Pandemic Serovars (O3:K6 and O4:K68) of Vibrio parahaemolyticus Associated with Diarrhea in Mozambique: Spread of the Pandemic into the African Continent

M. Ansaruzzaman,1* Marcelino Lucas,2 Jacqueline L. Deen,3 N. A. Bhuiyan,1 Xuan-Yi Wang,3 Ashrafus Safa,1 Marzia Sultana,1 A. Chowdhury,1 G. Balakrish Nair,1 David A. Sack,1 Lorenz von Seidlein,3 Mahesh K. Puri,3 Mohammad Ali,3 Claire-Lise Chaignat,4 John D. Clemens,3 and Avertino Barreto2

ICDDR,B, Mohakhali, Dhaka 1000, Bangladesh,1 Ministry of Health, Maputo, Mozambique,2 International Vaccine Institute, Seoul, Korea,3 World Health Organization, Geneva, Switzerland4

Received 9 November 2004/ Returned for modification 5 January 2005/ Accepted 24 February 2005

Forty-two episodes of Vibrio parahaemolyticus infections were detected in Beira, Mozambique, from January to May 2004. The majority of the isolates (81%) belonged to the pandemic serovars (O3:K6 and O4:K68) of V. parahaemolyticus. The pandemic serovars were positive by group-specific PCR (GS-PCR) and a PCR specific for open reading frame ORF8 (ORF8-PCR), which are molecular markers of the pandemic clone, and were positive for tdh but negative for trh. The remaining 19% of the strains also possessed the tdh gene but were GS-PCR and ORF8-PCR negative and did not belong to the pandemic serovars. Patients with V. parahaemolyticus infection were older (mean age, 27 years) than patients infected by other diarrheal agents (mean age, 21 years). Ten percent of diarrhea patients from whom no V. parahaemolyticus was cultured were severely dehydrated, but none of the V. parahaemolyticus cases were severely dehydrated. This is the first report of the isolation of pandemic strains of V. parahaemolyticus in sub-Saharan Africa and clearly indicates that the pandemic of V. parahaemolyticus has spread into the African continent.

* Corresponding author. Mailing address: Laboratory Sciences Division, ICDDR,B: Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh. Phone: 880 2 8811751. Fax: 880 2 8812529. E-mail: ansar{at}icddrb.org.

Journal of Clinical Microbiology, June 2005, p. 2559-2562, Vol. 43, No. 6
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.6.2559-2562.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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