Previous Article | Next Article 
Journal of Clinical Microbiology, June 2005, p. 2651-2655, Vol. 43, No. 6
0095-1137/05/$08.00+0 doi:10.1128/JCM.43.6.2651-2655.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Novel Chemically Modified Liquid Medium That Will Support the Growth of Seven Bartonella Species
Ricardo G. Maggi,
Ashlee W. Duncan, and
Edward B. Breitschwerdt*
Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606
Received 29 October 2004/
Returned for modification 24 December 2004/
Accepted 30 January 2005
Bacteria of the genus Bartonella, a member of the Alphaproteobacteria, are fastidious, gram-negative, aerobic bacilli that comprise numerous species, subspecies, and subtypes. In human and veterinary medicine, species isolation remains a vital component of the diagnostic and therapeutic management of Bartonella infection. We describe a novel, chemically modified, insect-based liquid culture medium that supports the growth of at least seven Bartonella species. This medium will also support cocultures consisting of different Bartonella species, and it facilitated the primary isolation of Bartonella henselae from blood and aqueous fluid of naturally infected cats. This liquid growth medium may provide an advantage over conventional direct blood agar plating for the diagnostic confirmation of bartonellosis.
* Corresponding author. Mailing address: Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606. Phone: (919) 513-8277. Fax: (919) 513-6336. E-mail:
ed_breitschwerdt{at}ncsu.edu.
Journal of Clinical Microbiology, June 2005, p. 2651-2655, Vol. 43, No. 6
0095-1137/05/$08.00+0 doi:10.1128/JCM.43.6.2651-2655.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Maggi, R. G., Raverty, S. A., Lester, S. J., Huff, D. G., Haulena, M., Ford, S. L., Nielsen, O., Robinson, J. H., Breitschwerdt, E. B.
(2008). BARTONELLA HENSELAE IN CAPTIVE AND HUNTER-HARVESTED BELUGA (DELPHINAPTERUS LEUCAS). J Wildl Dis
44: 871-877
[Abstract]
[Full Text]
-
Riess, T., Dietrich, F., Schmidt, K. V., Kaiser, P. O., Schwarz, H., Schafer, A., Kempf, V. A. J.
(2008). Analysis of a Novel Insect Cell Culture Medium-Based Growth Medium for Bartonella Species. Appl. Environ. Microbiol.
74: 5224-5227
[Abstract]
[Full Text]
-
Cadenas, M. B., Bradley, J., Maggi, R. G., Takara, M., Hegarty, B. C., Breitschwerdt, E. B.
(2008). Molecular Characterization of Bartonella vinsonii subsp. berkhoffii Genotype III. J. Clin. Microbiol.
46: 1858-1860
[Abstract]
[Full Text]
-
Kosoy, M., Morway, C., Sheff, K. W., Bai, Y., Colborn, J., Chalcraft, L., Dowell, S. F., Peruski, L. F., Maloney, S. A., Baggett, H., Sutthirattana, S., Sidhirat, A., Maruyama, S., Kabeya, H., Chomel, B. B., Kasten, R., Popov, V., Robinson, J., Kruglov, A., Petersen, L. R.
(2008). Bartonella tamiae sp. nov., a Newly Recognized Pathogen Isolated from Three Human Patients from Thailand. J. Clin. Microbiol.
46: 772-775
[Abstract]
[Full Text]
-
Garcia-Esteban, C., Gil, H., Rodriguez-Vargas, M., Gerrikagoitia, X., Barandika, J., Escudero, R., Jado, I., Garcia-Amil, C., Barral, M., Garcia-Perez, A. L., Bhide, M., Anda, P.
(2008). Molecular Method for Bartonella Species Identification in Clinical and Environmental Samples. J. Clin. Microbiol.
46: 776-779
[Abstract]
[Full Text]
-
Breitschwerdt, E. B., Maggi, R. G., Sigmon, B., Nicholson, W. L.
(2007). Isolation of Bartonella quintana from a Woman and a Cat following Putative Bite Transmission. J. Clin. Microbiol.
45: 270-272
[Abstract]
[Full Text]