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Journal of Clinical Microbiology, June 2005, p. 2651-2655, Vol. 43, No. 6
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.6.2651-2655.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Novel Chemically Modified Liquid Medium That Will Support the Growth of Seven Bartonella Species

Ricardo G. Maggi, Ashlee W. Duncan, and Edward B. Breitschwerdt*

Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606

Received 29 October 2004/ Returned for modification 24 December 2004/ Accepted 30 January 2005

Bacteria of the genus Bartonella, a member of the Alphaproteobacteria, are fastidious, gram-negative, aerobic bacilli that comprise numerous species, subspecies, and subtypes. In human and veterinary medicine, species isolation remains a vital component of the diagnostic and therapeutic management of Bartonella infection. We describe a novel, chemically modified, insect-based liquid culture medium that supports the growth of at least seven Bartonella species. This medium will also support cocultures consisting of different Bartonella species, and it facilitated the primary isolation of Bartonella henselae from blood and aqueous fluid of naturally infected cats. This liquid growth medium may provide an advantage over conventional direct blood agar plating for the diagnostic confirmation of bartonellosis.


* Corresponding author. Mailing address: Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606. Phone: (919) 513-8277. Fax: (919) 513-6336. E-mail: ed_breitschwerdt{at}ncsu.edu.


Journal of Clinical Microbiology, June 2005, p. 2651-2655, Vol. 43, No. 6
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.6.2651-2655.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.




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