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Journal of Clinical Microbiology, June 2005, p. 2756-2763, Vol. 43, No. 6
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.6.2756-2763.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Central European Dobrava Hantavirus Isolate from a Striped Field Mouse (Apodemus agrarius)

Boris Klempa,1,2 Michal Stanko,3 Milan Labuda,3 Rainer Ulrich,1,{dagger} Helga Meisel,1 and Detlev H. Krüger1*

Institute of Virology, Helmut Ruska Haus, Charité School of Medicine, Humboldt University, D-10098 Berlin, Germany,1 Institute of Virology,2 Institute of Zoology, Slovak Academy of Sciences, 84206 Bratislava, Slovakia3

Received 16 September 2004/ Returned for modification 6 November 2004/ Accepted 20 February 2005

Dobrava virus (DOBV) is a hantavirus that causes hemorrhagic fever with renal syndrome (HFRS) in Europe. It is hosted by at least two rodent species, Apodemus flavicollis and A. agrarius. According to their natural hosts they form the distinct genetic lineages DOBV-Af and DOBV-Aa, respectively. We have now established a DOBV isolate named Slovakia (SK/Aa) from an A. agrarius animal captured in Slovakia. The complete S and M and partial L segment nucleotide sequences of the new isolate were determined. Phylogenetic analyses showed that the SK/Aa isolate clustered together with the other DOBV-Aa sequences amplified from A. agrarius before and can be taken as the representative of this genetic lineage. SK/Aa, in comparison with a DOBV-Af isolate, was used for serotyping neutralizing antibodies of HFRS patients in Central Europe. Most patients' sera exhibited a higher endpoint titer when probed with our new isolate, suggesting that DOBV-Aa strains are responsible for most of the DOBV-caused HFRS cases in this region.

* Corresponding author. Institute of Virology, Helmut Ruska Haus, Charité School of Medicine, Humboldt University, D-10098 Berlin, Germany. Phone: 49-30-450-525-092. Fax: 49-30-450-525-907. E-mail: detlev.kruger{at}charite.de.

{dagger} Present address: Friedrich-Loeffler-Institut, Bundesforschungsinstitut für Tiergesundheit, Institut für Epidemiologie, D-16868 Wusterhausen, Germany.

Journal of Clinical Microbiology, June 2005, p. 2756-2763, Vol. 43, No. 6
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.6.2756-2763.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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