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Journal of Clinical Microbiology, June 2005, p. 2909-2912, Vol. 43, No. 6
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.6.2909-2912.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Multicenter Evaluation of a Candida albicans Peptide Nucleic Acid Fluorescent In Situ Hybridization Probe for Characterization of Yeast Isolates from Blood Cultures

D. A. Wilson,1 M. J. Joyce,2 L. S. Hall,3 L. B. Reller,2 G. D. Roberts,3 G. S. Hall,1 B. D. Alexander,2 and G. W. Procop1*

Section of Clinical Microbiology, The Cleveland Clinic Foundation, Cleveland, Ohio,1 Clinical Microbiology Laboratory, Duke University Medical Center, Durham, North Carolina,2 Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota3

Received 29 October 2004/ Returned for modification 20 December 2004/ Accepted 6 January 2005

We evaluated aliquots from 244 clinical blood culture bottles that demonstrated yeasts on Gram stain using a Candida albicans peptide nucleic acid (PNA) fluorescent in situ hybridization (FISH) probe. The sensitivity, specificity, positive predictive value, and negative predictive value of the C. albicans PNA FISH test in this study were 99%, 100%, 100%, and 99.3%, respectively.

* Corresponding author. Mailing address: Clinical Microbiology/L10, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195. Phone: (216) 444-5879. Fax: (216) 445-6984. E-mail: procopg{at}ccf.org.

Journal of Clinical Microbiology, June 2005, p. 2909-2912, Vol. 43, No. 6
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.6.2909-2912.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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