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Journal of Clinical Microbiology, June 2005, p. 2932-2933, Vol. 43, No. 6
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.6.2932-2933.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Shortcut Method for Extraction of Staphylococcus aureus DNA from Blood Cultures and Conventional Cultures for Use in Real-Time PCR Assays

D. Nicholas Adams*

Department of Microbiology, Royal Prince Alfred Hospital, Missenden Rd., Camperdown 2050, NSW, Australia

Received 1 February 2005/ Accepted 7 February 2005

PCR allows rapid determination of both the presence of Staphylococcus aureus and of methicillin resistance. I present here a simple method for direct extraction from blood cultures or colonies for use in real-time PCR, eliminating tedious, time-consuming methods of DNA extraction which may otherwise deter microbiology labs from offering PCR.

* Mailing address: Department of Microbiology, Royal Prince Alfred Hospital, Missenden Rd., Camperdown 2050, NSW, Australia. Phone: 61 2 95158278. Fax: 61-2 95155235. E-mail: nick.adams{at}email.cs.nsw.gov.au.

Journal of Clinical Microbiology, June 2005, p. 2932-2933, Vol. 43, No. 6
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.6.2932-2933.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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