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Journal of Clinical Microbiology, July 2005, p. 3110-3113, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3110-3113.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

AmpC Disk Test for Detection of Plasmid-Mediated AmpC ß-Lactamases in Enterobacteriaceae Lacking Chromosomal AmpC ß-Lactamases

Jennifer A. Black, Ellen Smith Moland, and Kenneth S. Thomson*

Center for Research in Antiinfectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska

Received 22 February 2005/ Returned for modification 7 March 2005/ Accepted 14 March 2005

Although plasmid-mediated AmpC ß-lactamases were first reported in the late 1980s, many infectious disease personnel remain unaware of their clinical importance. These enzymes are typically produced by isolates of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. and are associated with multiple antibiotic resistance that leaves few therapeutic options. Plasmid-mediated AmpC ß-lactamases have been associated with false in vitro susceptibility to cephalosporins. Many laboratories do not test for this resistance mechanism because current tests are inconvenient, subjective, lack sensitivity and/or specificity, or require reagents that are not readily available. In this study a new test, the AmpC disk test, based on filter paper disks impregnated with EDTA, was found to be a highly sensitive, specific, and convenient means of detection of plasmid-mediated AmpC ß-lactamases in organisms lacking a chromosomally mediated AmpC ß-lactamase. Using cefoxitin insusceptibility as a screen, the test accurately distinguished AmpC and extended-spectrum ß-lactamase production and differentiated AmpCs from non-ß-lactamase mechanisms of cefoxitin insusceptibility, such as reduced outer membrane permeability. The test is a potentially useful diagnostic tool. It can provide important infection control information and help to ensure that infected patients receive appropriate antibiotic therapy.

* Corresponding author. Mailing address: Center for Research in Antiinfectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178. Phone: (402) 280-2921. Fax: (402) 280-1875. E-mail: kstaac{at}creighton.edu.

Journal of Clinical Microbiology, July 2005, p. 3110-3113, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3110-3113.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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