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Journal of Clinical Microbiology, July 2005, p. 3136-3139, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3136-3139.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Real-Time Nucleic Acid Sequence-Based Amplification Using Molecular Beacons for Detection of Enterovirus RNA in Clinical Specimens

Marie L. Landry,1,2* Robin Garner,2 and David Ferguson1,2

Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06520,1 Clinical Virology Laboratory, Yale New Haven Hospital, New Haven, Connecticut 065042

Received 16 February 2005/ Returned for modification 28 March 2005/ Accepted 6 April 2005

Real-time nucleic acid sequence-based amplification (NASBA) using molecular beacon technology (NASBA-beacon) was compared to standard NASBA with postamplification hybridization using electrochemiluminescently labeled probes (NASBA-ECL) for detection of enteroviruses (EV) in 133 cerebrospinal fluid and 27 stool samples. NASBA-ECL and NASBA-beacon were similar in sensitivity, detecting 55 (100%) and 52 (94.5%) EV-positive samples, respectively. There were no false positives. Both NASBA assays were significantly more sensitive than culture. Real-time NASBA-beacon reagents and equipment rental were more expensive than those for NASBA-ECL; however, time to result was shortened by 1.5 h, hands-on time was reduced by 25 min, and the assay was much simpler for technologists to learn and perform.

* Corresponding author. Mailing address: Department of Laboratory Medicine, P.O. Box 208035, Yale University School of Medicine, New Haven, CT 06520-8035. Phone: (203) 688-3475. Fax: (203) 688-8177. E-mail: marie.landry{at}yale.edu.

Journal of Clinical Microbiology, July 2005, p. 3136-3139, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3136-3139.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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