This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chibo, D.
Right arrow Articles by Birch, C. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chibo, D.
Right arrow Articles by Birch, C. J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 2005, p. 3145-3149, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3145-3149.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Applicability of Oral Fluid Collected onto Filter Paper for Detection and Genetic Characterization of Measles Virus Strains

Doris Chibo,* Michaela A. Riddell, Mike G. Catton, and Chris J. Birch

WHO Regional Measles Reference Laboratory for the Western Pacific Region, Victorian Infectious Diseases Reference Laboratory, North Melbourne, 3051 Victoria, Australia

Received 6 June 2004/ Returned for modification 9 November 2004/ Accepted 9 March 2005

Expansion of measles molecular surveillance to developing countries where measles is endemic will help facilitate measles control. Limited infrastructure in these areas is a barrier to referral of specimens suitable for measles virus (MV) genotyping. In this study, we demonstrate that oral fluid dried onto filter paper can be used for the detection and characterization of MV strains. Using this approach, an MV-positive sample by reverse transcriptase PCR could be obtained from 67% of serologically confirmed acute measles cases. Mimicking certain environmental conditions and duration of transportation established that MV RNA remained detectable and suitable for nucleic acid sequencing in oral fluid spots for at least 1 week. In the context of a measles outbreak in a remote region of the world where infrastructure is poor, oral fluid samples dried onto filter paper and sent to a specialized laboratory for testing will aid in the identification and characterization of the causative MV strain.

* Corresponding author. Mailing address: 10 Wreckyn St., North Melbourne 3051, Victoria, Australia. Phone: 61 3 9342 2627. Fax: 61 3 9342 2666. E-mail: Doris.chibo{at}mh.org.au.

Journal of Clinical Microbiology, July 2005, p. 3145-3149, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3145-3149.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lenselink, C. H., de Bie, R. P., van Hamont, D., Bakkers, J. M. J. E., Quint, W. G. V., Massuger, L. F. A. G., Bekkers, R. L. M., Melchers, W. J. G. (2009). Detection and Genotyping of Human Papillomavirus in Self-Obtained Cervicovaginal Samples by Using the FTA Cartridge: New Possibilities for Cervical Cancer Screening. J. Clin. Microbiol. 47: 2564-2570 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»