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Journal of Clinical Microbiology, July 2005, p. 3304-3308, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3304-3308.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of a Locked-Nucleic-Acid Oligomer in the Clamped-Probe Assay for Detection of a Minority Pfcrt K76T Mutant Population of Plasmodium falciparum

Alice Senescau,1,{dagger} Antoine Berry,1*,{dagger} Françoise Benoit-Vical,1,2 Olfert Landt,3 Richard Fabre,1 Joël Lelièvre,1,2 Sophie Cassaing,1 and Jean-François Magnaval1

Department of Parasitology, Rangueil University Hospital, 31059 Toulouse 9, France,1 Laboratoire de Chimie de Coordination du CNRS, 205 route de Narbonne, 31077 Toulouse 4, France,2 Tib Molbiol, Tempelhofer weg 11-12, D-10829 Berlin, Germany3

Received 11 September 2004/ Returned for modification 20 October 2004/ Accepted 31 March 2005

Given the emergence of drug resistance and the high rate of polyclonal microorganism infections, the availability of a fast and sensitive test to detect minority mutant populations would be an improvement in the diagnosis of infectious diseases. A clamped-probe real-time PCR assay to diagnose the Plasmodium falciparum K76T mutation in clone populations was developed, using a wild-type-specific locked-nucleic-acid-containing oligomer to suppress wild-type PCR amplification and to enhance melting analysis with a mutation-specific detection probe.

* Corresponding author. Mailing address: Service de Parasitologie, CHU Rangueil, TSA 50032, 31059 Toulouse 9, France. Phone: 33-5-61-32-32-05. Fax: 33-5-61-32-22-30. E-mail: berry.a{at}chu-toulouse.fr.

{dagger} A.S. and A.B. contributed equally to this study.

Journal of Clinical Microbiology, July 2005, p. 3304-3308, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3304-3308.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rupp, J., Solbach, W., Gieffers, J. (2006). Single-Nucleotide-Polymorphism-Specific PCR for Quantification and Discrimination of Chlamydia pneumoniae Genotypes by Use of a "Locked" Nucleic Acid.. Appl. Environ. Microbiol. 72: 3785-3787 [Abstract] [Full Text]  


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