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Journal of Clinical Microbiology, July 2005, p. 3309-3313, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3309-3313.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Countrywide Spread of Community- and Hospital-Acquired Extended-Spectrum ß-Lactamase (CTX-M-15)-Producing Enterobacteriaceae in Lebanon

Carole Moubareck,1 Ziad Daoud,2 Noha I. Hakimé,2 Monzer Hamzé,3 Nicole Mangeney,1 Hiam Matta,4 Jacques E. Mokhbat,5 Raymond Rohban,6 Dolla Karam Sarkis,7 and Florence Doucet-Populaire1,8*

Laboratoire de Microbiologie, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris 5-René Descartes, Paris, France,1 Saint George Hospital, and University of Balamand, Beirut, Lebanon,2 Lebanese University, Beirut, and Nini Hospital, Tripoli, Lebanon,3 Arz Hospital, Al Metn, Lebanon,4 Rizk Hospital, Beirut, Lebanon,5 Saint Joseph Hospital, Beirut, Lebanon,6 Hôtel Dieu de France Hospital, Beirut, Lebanon,7 C. H. de Versailles, Le Chesnay, France8

Received 17 January 2005/ Returned for modification 25 February 2005/ Accepted 4 April 2005

A prospective study was carried out to assess the extent of carriage of extended-spectrum ß-lactamase (ESBL)-producing Enterobacteriaceae at both hospital and community levels in Lebanon. A total of 1,442 fecal samples were collected from hospital-based patients and 58 from health care workers of six Lebanese tertiary care general hospitals located in different areas of Lebanon between January and March 2003. A total of 382 fecal samples were also collected from healthy subjects between April and June 2003. The samples analysis led to the identification of 118 strains as ESBL producers based on the synergistic effects between clavulanate and selected ß-lactams (ceftazidime and cefotaxime). These strains were isolated from 72 subjects: 61 patients, 2 health care workers, and 9 healthy subjects. One representative strain per subject was selected, and a total of 72 nonduplicate ESBL producers, including a high majority of Escherichia coli (n = 56), Klebsiella pneumoniae (n = 9), Enterobacter cloacae (n = 6), and Citrobacter freundii (n = 1), were characterized. The molecular analysis revealed that the majority of the strains (83%) express CTX-M-15 ESBL (pI 8.6). SHV-5a ESBL (pI 8.2) was produced by 18% of the strains. DNA macrorestriction analysis of ESBL-producing E. coli presented 38 different genotypes, revealing the absence of clonal link among these strains. In addition to the fact that the present study highlights the emergence and the countrywide dissemination of CTX-M-15-producing E. coli in Lebanon, it represents the first report of an SHV-5a-producing C. freundii.

* Corresponding author. Mailing address: Faculté des Sciences Pharmaceutiques et Biologiques, Laboratoire de Microbiologie, 4 Av. de l'Observatoire, 75270 Paris Cedex 06, France. Phone: (33) 1-53-73-99-13. Fax: (33) 1-53-73-99-23. E-mail: florence.doucet-populaire{at}univ-paris5.fr.

Journal of Clinical Microbiology, July 2005, p. 3309-3313, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3309-3313.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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