This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Francois, P.
Right arrow Articles by Schrenzel, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Francois, P.
Right arrow Articles by Schrenzel, J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 2005, p. 3346-3355, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3346-3355.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of an Automated Multiple-Locus, Variable-Number Tandem Repeat-Based Method for Rapid and High-Throughput Genotyping of Staphylococcus aureus Isolates

Patrice Francois,1* Antoine Huyghe,1 Yvan Charbonnier,1 Manuela Bento,1 Sébastien Herzig,2 Ivan Topolski,1 Bénédicte Fleury,3 Daniel Lew,3 Pierre Vaudaux,3 Stephan Harbarth,3,4 Willem van Leeuwen,5 Alex van Belkum,5 Dominique S. Blanc,6 Didier Pittet,3,4 and Jacques Schrenzel1,2

Genomic Research Laboratory,1 Clinical Microbiology Laboratory,2 Service of Infectious Diseases,3 Infection Control Program, University of Geneva Hospitals, CH-1211 Geneva 14, Switzerland,4 Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands,5 Hospital of Preventive Medicine, University Hospital of Lausanne, CH-1011 Lausanne, Switzerland6

Received 15 September 2004/ Returned for modification 5 November 2004/ Accepted 14 March 2005

Fast and reliable genotyping methods that allow real-time epidemiological surveillance would be instrumental to monitoring of the spread of methicillin-resistant Staphylococcus aureus. We describe an automated variable-number tandem repeat-based method for the rapid genotyping of Staphylococcus aureus. Multiplex PCR amplifications with eight primer pairs that target gene regions with variable numbers of tandem repeats were resolved by microcapillary electrophoresis and automatically assessed by cluster analysis. This genotyping technique was evaluated for its discriminatory power and reproducibility with clinical isolates of various origins, including a panel of control strains previously characterized by several typing methods and collections from either long-term carriers or defined nosocomial outbreaks. All steps of this new procedure were developed to ensure a rapid turnaround time and moderate cost. The results obtained suggest that this rapid approach is a valuable tool for the genotyping of S. aureus isolates in real time.

* Corresponding author. Mailing address: University of Geneva Hospitals, Service of Infectious Diseases/Genomic Research Laboratory, CH-1211 Geneva 14, Switzerland. Phone: (41)-22 3729338. Fax: (41)-22 3729830. E-mail:patrice.francois{at}genomic.ch.

Journal of Clinical Microbiology, July 2005, p. 3346-3355, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3346-3355.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pourcel, C., Hormigos, K., Onteniente, L., Sakwinska, O., Deurenberg, R. H., Vergnaud, G. (2009). Improved Multiple-Locus Variable-Number Tandem-Repeat Assay for Staphylococcus aureus Genotyping, Providing a Highly Informative Technique Together with Strong Phylogenetic Value. J. Clin. Microbiol. 47: 3121-3128 [Abstract] [Full Text]  
  • Rasschaert, G., Vanderhaeghen, W., Dewaele, I., Janez, N., Huijsdens, X., Butaye, P., Heyndrickx, M. (2009). Comparison of Fingerprinting Methods for Typing Methicillin-Resistant Staphylococcus aureus Sequence Type 398. J. Clin. Microbiol. 47: 3313-3322 [Abstract] [Full Text]  
  • van der Mee-Marquet, N., Francois, P., Domelier, A.-S., Arnault, L., Girard, N., Schrenzel, J., Quentin, R., and the Bloodstream Infection Study Group of the R, (2009). Variable-Number Tandem Repeat Analysis and Multilocus Sequence Typing Data Confirm the Epidemiological Changes Observed with Staphylococcus aureus Strains Isolated from Bloodstream Infections. J. Clin. Microbiol. 47: 2863-2871 [Abstract] [Full Text]  
  • Conceicao, T., Aires de Sousa, M., de Lencastre, H. (2009). Staphylococcal Interspersed Repeat Unit Typing of Staphylococcus aureus: Evaluation of a New Multilocus Variable-Number Tandem-Repeat Analysis Typing Method. J. Clin. Microbiol. 47: 1300-1308 [Abstract] [Full Text]  
  • Ikawaty, R., Willems, R. J. L., Box, A. T. A., Verhoef, J., Fluit, A. C. (2008). Novel Multiple-Locus Variable-Number Tandem-Repeat Analysis Method for Rapid Molecular Typing of Human Staphylococcus aureus. J. Clin. Microbiol. 46: 3147-3151 [Abstract] [Full Text]  
  • Collery, M. M., Smyth, D. S., Twohig, J. M., Shore, A. C., Coleman, D. C., Smyth, C. J. (2008). Molecular typing of nasal carriage isolates of Staphylococcus aureus from an Irish university student population based on toxin gene PCR, agr locus types and multiple locus, variable number tandem repeat analysis. J Med Microbiol 57: 348-358 [Abstract] [Full Text]  
  • Broza, Y. Y., Danin-Poleg, Y., Lerner, L., Broza, M., Kashi, Y. (2007). Vibrio vulnificus Typing Based on Simple Sequence Repeats: Insights into the Biotype 3 Group. J. Clin. Microbiol. 45: 2951-2959 [Abstract] [Full Text]  
  • Tenover, F. C., Vaughn, R. R., McDougal, L. K., Fosheim, G. E., McGowan, J. E. Jr. (2007). Multiple-Locus Variable-Number Tandem-Repeat Assay Analysis of Methicillin-Resistant Staphylococcus aureus Strains. J. Clin. Microbiol. 45: 2215-2219 [Abstract] [Full Text]  
  • Francois, P., Bento, M., Renzi, G., Harbarth, S., Pittet, D., Schrenzel, J. (2007). Evaluation of Three Molecular Assays for Rapid Identification of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 45: 2011-2013 [Abstract] [Full Text]  
  • Ghebremedhin, B., Konig, W., Witte, W., Hardy, K. J., Hawkey, P. M., Konig, B. (2007). Subtyping of ST22-MRSA-IV (Barnim epidemic MRSA strain) at a university clinic in Germany from 2002 to 2005. J Med Microbiol 56: 365-375 [Abstract] [Full Text]  
  • Kuhn, G., Francioli, P., Blanc, D. S. (2007). Double-Locus Sequence Typing Using clfB and spa, a Fast and Simple Method for Epidemiological Typing of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 45: 54-62 [Abstract] [Full Text]  
  • Huygens, F., Inman-Bamber, J., Nimmo, G. R., Munckhof, W., Schooneveldt, J., Harrison, B., McMahon, J. A., Giffard, P. M. (2006). Staphylococcus aureus Genotyping Using Novel Real-Time PCR Formats.. J. Clin. Microbiol. 44: 3712-3719 [Abstract] [Full Text]  
  • Sabat, A., Malachowa, N., Miedzobrodzki, J., Hryniewicz, W. (2006). Comparison of PCR-Based Methods for Typing Staphylococcus aureus Isolates.. J. Clin. Microbiol. 44: 3804-3807 [Abstract] [Full Text]  
  • Strommenger, B., Kettlitz, C., Weniger, T., Harmsen, D., Friedrich, A. W., Witte, W. (2006). Assignment of Staphylococcus Isolates to Groups by spa Typing, SmaI Macrorestriction Analysis, and Multilocus Sequence Typing.. J. Clin. Microbiol. 44: 2533-2540 [Abstract] [Full Text]  
  • Francois, P., Koessler, T., Huyghe, A., Harbarth, S., Bento, M., Lew, D., Etienne, J., Pittet, D., Schrenzel, J. (2006). Rapid Staphylococcus aureus agr Type Determination by a Novel Multiplex Real-Time Quantitative PCR Assay.. J. Clin. Microbiol. 44: 1892-1895 [Abstract] [Full Text]  
  • Koessler, T., Francois, P., Charbonnier, Y., Huyghe, A., Bento, M., Dharan, S., Renzi, G., Lew, D., Harbarth, S., Pittet, D., Schrenzel, J. (2006). Use of Oligoarrays for Characterization of Community-Onset Methicillin-Resistant Staphylococcus aureus.. J. Clin. Microbiol. 44: 1040-1048 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»