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Journal of Clinical Microbiology, July 2005, p. 3522-3525, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3522-3525.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Isolation of an Atypical Strain of Actinobacillus pleuropneumoniae Serotype 1 with a Truncated Lipopolysaccharide Outer Core and No O-Antigen

Mario Jacques,1,6* Josée Labrie,1,6 Frank St. Michael,2 Andrew D. Cox,2 Marie-Anne Paradis,3 C. Paul Dick,3 Christian Klopfenstein,4 André Broes,5 Nahuel Fittipaldi,1,6 and Marcelo Gottschalk1,6

Groupe de recherche sur les maladies infectieuses du porc, Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada J2S 7C6,1 Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada K1A OR6,2 Elanco, Division of Eli Lilly and Co., Guelph, Ontario, Canada N1G 4T2,3 Centre de développement du porc du Québec inc., Sainte-Foy, Québec, Canada G1V 4M7,4 Génétiporc Inc., St-Bernard, Québec, Canada G0S 2G0,5 Canadian Research Network on Bacterial Pathogens of Swine, St-Hyacinthe, Quebec, Canada J25-7C66

Received 3 November 2004/ Returned for modification 19 December 2004/ Accepted 27 February 2005

A field isolate of Actinobacillus pleuropneumoniae, the causative agent of porcine fibrinohemorrhagic necrotizing pleuropneumonia, was sent to the diagnostic laboratory for serotyping. The isolate presented a clear reaction, with both polyclonal antibodies against serotype 1 and monoclonal antibodies against the capsular polysaccharide of serotype 1. It also exhibited a PCR profile of Apx toxins expected for serotype 1. The isolate, however, failed to react with monoclonal antibodies against the O-antigen of serotype 1 lipopolysaccharide (LPS), suggesting a rough phenotype. The lipid A-core region of the isolate migrated faster than the corresponding region of the serotype 1 reference strain S4074 by Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis, suggesting the presence of a truncated core. Sugar analysis and mass spectrometry analysis of the O-deacylated LPS from the field isolate were consistent with the absence of O-antigen and truncation of the outer core compared to the wild-type reference strain. Experimental infection of pigs confirmed the virulence of the isolate. This is the first report of an isolate of A. pleuropneumoniae serotype 1 with a truncated outer core and a rough LPS phenotype. Veterinary diagnostic laboratories should be vigilant, since infections caused by such an isolate will not be detected by serological tests based on LPS O-antigen.

* Corresponding author. Mailing address: Groupe de recherche sur les maladies infectieuses du porc, Faculté de médecine vétérinaire, Université de Montréal, 3200 rue Sicotte, C.P. 5000, St-Hyacinthe, Québec, Canada J2S 7C6. Phone: (450) 773-8521, ext. 18348. Fax: (450) 778-8108. E-mail: mario.jacques{at}umontreal.ca.

Journal of Clinical Microbiology, July 2005, p. 3522-3525, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3522-3525.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Auger, E., Deslandes, V., Ramjeet, M., Contreras, I., Nash, J. H. E., Harel, J., Gottschalk, M., Olivier, M., Jacques, M. (2009). Host-Pathogen Interactions of Actinobacillus pleuropneumoniae with Porcine Lung and Tracheal Epithelial Cells. Infect. Immun. 77: 1426-1441 [Abstract] [Full Text]  


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