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Journal of Clinical Microbiology, July 2005, p. 3533-3536, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3533-3536.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of Vibrio parahaemolyticus Pandemic Group-Specific DNA Sequence by Genomic Subtraction

Masatoshi Okura,1 Ro Osawa,1* Eiji Arakawa,2 Jun Terajima,2 and Haruo Watanabe2

Department of Bioscience, Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe, 657-8501, Japan,1 Department of Bacteriology, National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan2

Received 6 January 2005/ Returned for modification 18 February 2005/ Accepted 28 February 2005

A genomic subtraction between a pandemic Vibrio parahaemolyticus and a nonpandemic strain that seemed to be clonally related was performed. A subtractive DNA fragment was identified to be a part of a 16-kbp insertion sequence which was present in almost all pandemic strains but not in nonpandemic strains tested.


* Corresponding author. Mailing address: Department of Bioscience, Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe City, Japan 657-8501. Phone: 81-78-803-5804. Fax: 81-78-803-5804. E-mail: osawa{at}ans.kobe-u.ac.jp.


Journal of Clinical Microbiology, July 2005, p. 3533-3536, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3533-3536.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.




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