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Journal of Clinical Microbiology, August 2005, p. 3699-3703, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.3699-3703.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of the Genotype MTBDR Assay for Rapid Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis Complex Isolates

Doris Hillemann,1* Michael Weizenegger,2 Tanja Kubica,1 Elvira Richter,1 and Stefan Niemann1

Forschungszentrum Borstel, National Reference Center for Mycobacteria, D-23845 Borstel, Germany,1 Department of Microbiology, Limbach Laboratory, D-69126 Heidelberg, Germany2

Received 1 February 2005/ Returned for modification 1 March 2005/ Accepted 19 April 2005

A commercially available DNA strip assay (Genotype MTBDR; Hain Lifescience, Nehren, Germany) was evaluated for its ability to detect mutations conferring resistance to rifampin (RMP) and isoniazid (INH) in clinical Mycobacterium tuberculosis complex isolates. A total of 103 multidrug-resistant (MDR; i.e., at least resistant to RMP and INH) and 40 fully susceptible strains isolated in Germany in 2001 in which resistance mutations have been previously defined by DNA sequencing and real-time PCR analysis were investigated. The Genotype MTBDR assay identified 102 of the 103 MDR strains with mutations in the rpoB gene (99%) and 91 strains (88.4%) with mutations in codon 315 of katG. All 40 susceptible strains showed a wild-type MTBDR hybridization pattern. The concordance between the MTBDR assay and the DNA sequencing results was 100%. Compared to conventional drug susceptibility testing, the sensitivity and specificity were 99 and 100% for RMP resistance and 88.4 and 100% for INH resistance, respectively. In conclusion, the MTBDR assay is a rapid and easy-to-perform test for the detection of the most common mutations found in MDR M. tuberculosis strains that can readily be included in a routine laboratory work flow.

* Corresponding author. Mailing address: Forschungszentrum Borstel, National Reference Center for Mycobacteria, Parkallee 18, D-23845 Borstel, Germany. Phone: (49) 4537-188761. Fax: (49) 4537-188311. E-mail: sniemann{at}fz-borstel.de.

Journal of Clinical Microbiology, August 2005, p. 3699-3703, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.3699-3703.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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