This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Luan, S.-L.
Right arrow Articles by Norgren, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Luan, S.-L.
Right arrow Articles by Norgren, M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2005, p. 3727-3733, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.3727-3733.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Multilocus Sequence Typing of Swedish Invasive Group B Streptococcus Isolates Indicates a Neonatally Associated Genetic Lineage and Capsule Switching

Shi-Lu Luan,1* Margareta Granlund,2 Mats Sellin,2 Teresa Lagergård,3 Brian G. Spratt,4 and Mari Norgren1

Department of Biomedical Laboratory Science, Umeå University, Umeå,1 Department of Clinical Bacteriology, Umeå University, Umeå,2 Department of Microbiology and Immunology, Gothenburg University, Gothenburg, Sweden,3 Department of Infectious Disease Epidemiology, Faculty of Medicine, Imperial College, St. Mary's Hospital, London, United Kingdom4

Received 17 February 2005/ Returned for modification 15 April 1005/ Accepted 11 May 2005

Streptococcus agalactiae, also designated group B streptococcus (GBS), is an important pathogen in neonates, pregnant women, and nonpregnant adults with predisposing conditions. We used multilocus sequence typing (MLST) to characterize 158 GBS isolates that were associated with neonatal and adult invasive disease and that were collected in northern and western Sweden from 1988 to 1997. Five major genetic lineages (sequence type [ST] 19, ST-17, ST-1, ST-23, and ST-9 complexes) were identified among the isolates, including serotype Ia, Ib, and II to V isolates, indicating a highly clonal population structure among invasive GBS isolates. A number of STs were found to contain isolates of different serotypes, which indicates that capsule switching occurred rather frequently. Two distantly related genetic lineages were identified among isolates of serotype III, namely, clonal complex 19 (CC19), and CC17. CC19 was equally common among isolates from adult and neonatal disease (accounting for 10.3% of GBS isolates from adult disease and 18.7% from neonatal disease), whereas CC17 significantly appeared to be associated with neonatal invasive disease (isolated from 21.9% of neonatal isolates but only 2.6% of adult isolates). The distribution of the mobile elements GBSi1 and IS1548 reveals that they can act as genetic markers for lineages CC17 and CC19, respectively.

* Corresponding author. Mailing address: Department of Clinical Bacteriology, Umeå University, S-90 185 Umeå, Sweden. Phone: 46-90-785 23 47. Fax: 46-90-785 22 25. E-mail: shilu.luan{at}climi.umu.se.

Journal of Clinical Microbiology, August 2005, p. 3727-3733, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.3727-3733.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Springman, A. C., Lacher, D. W., Wu, G., Milton, N., Whittam, T. S., Davies, H. D., Manning, S. D. (2009). Selection, Recombination, and Virulence Gene Diversity among Group B Streptococcal Genotypes. J. Bacteriol. 191: 5419-5427 [Abstract] [Full Text]  
  • Doro, F., Liberatori, S., Rodriguez-Ortega, M. J., Rinaudo, C. D., Rosini, R., Mora, M., Scarselli, M., Altindis, E., D'Aurizio, R., Stella, M., Margarit, I., Maione, D., Telford, J. L., Norais, N., Grandi, G. (2009). Surfome Analysis as a Fast Track to Vaccine Discovery: IDENTIFICATION OF A NOVEL PROTECTIVE ANTIGEN FOR GROUP B STREPTOCOCCUS HYPERVIRULENT STRAIN COH1. Mol. Cell. Proteomics 8: 1728-1737 [Abstract] [Full Text]  
  • Manning, S. D., Springman, A. C., Lehotzky, E., Lewis, M. A., Whittam, T. S., Davies, H. D. (2009). Multilocus Sequence Types Associated with Neonatal Group B Streptococcal Sepsis and Meningitis in Canada. J. Clin. Microbiol. 47: 1143-1148 [Abstract] [Full Text]  
  • Brochet, M., Couve, E., Bercion, R., Sire, J.-M., Glaser, P. (2009). Population Structure of Human Isolates of Streptococcus agalactiae from Dakar and Bangui. J. Clin. Microbiol. 47: 800-803 [Abstract] [Full Text]  
  • Evans, J. J., Bohnsack, J. F., Klesius, P. H., Whiting, A. A., Garcia, J. C., Shoemaker, C. A., Takahashi, S. (2008). Phylogenetic relationships among Streptococcus agalactiae isolated from piscine, dolphin, bovine and human sources: a dolphin and piscine lineage associated with a fish epidemic in Kuwait is also associated with human neonatal infections in Japan. J Med Microbiol 57: 1369-1376 [Abstract] [Full Text]  
  • Brochet, M., Rusniok, C., Couve, E., Dramsi, S., Poyart, C., Trieu-Cuot, P., Kunst, F., Glaser, P. (2008). Shaping a bacterial genome by large chromosomal replacements, the evolutionary history of Streptococcus agalactiae. Proc. Natl. Acad. Sci. USA 105: 15961-15966 [Abstract] [Full Text]  
  • Dahesh, S., Hensler, M. E., Van Sorge, N. M., Gertz, R. E. Jr., Schrag, S., Nizet, V., Beall, B. W. (2008). Point Mutation in the Group B Streptococcal pbp2x Gene Conferring Decreased Susceptibility to {beta}-Lactam Antibiotics. Antimicrob. Agents Chemother. 52: 2915-2918 [Abstract] [Full Text]  
  • Bohnsack, J. F., Whiting, A., Gottschalk, M., Dunn, D. M., Weiss, R., Azimi, P. H., Philips, J. B. III, Weisman, L. E., Rhoads, G. G., Lin, F.-Y. C. (2008). Population Structure of Invasive and Colonizing Strains of Streptococcus agalactiae from Neonates of Six U.S. Academic Centers from 1995 to 1999. J. Clin. Microbiol. 46: 1285-1291 [Abstract] [Full Text]  
  • Martins, E. R., Florindo, C., Martins, F., Aldir, I., Borrego, M. J., Brum, L., Ramirez, M., Melo-Cristino, J. (2007). Streptococcus agalactiae Serotype Ib as an Agent of Meningitis in Two Adult Nonpregnant Women. J. Clin. Microbiol. 45: 3850-3852 [Abstract] [Full Text]  
  • Martins, E. R., Melo-Cristino, J., Ramirez, M. (2007). Reevaluating the Serotype II Capsular Locus of Streptococcus agalactiae. J. Clin. Microbiol. 45: 3384-3386 [Abstract] [Full Text]  
  • Slotved, H.-C., Kong, F., Lambertsen, L., Sauer, S., Gilbert, G. L. (2007). Serotype IX, a Proposed New Streptococcus agalactiae Serotype. J. Clin. Microbiol. 45: 2929-2936 [Abstract] [Full Text]  
  • Gherardi, G., Imperi, M., Baldassarri, L., Pataracchia, M., Alfarone, G., Recchia, S., Orefici, G., Dicuonzo, G., Creti, R. (2007). Molecular Epidemiology and Distribution of Serotypes, Surface Proteins, and Antibiotic Resistance among Group B Streptococci in Italy. J. Clin. Microbiol. 45: 2909-2916 [Abstract] [Full Text]  
  • Hery-Arnaud, G., Bruant, G., Lanotte, P., Brun, S., Picard, B., Rosenau, A., van der Mee-Marquet, N., Rainard, P., Quentin, R., Mereghetti, L. (2007). Mobile Genetic Elements Provide Evidence for a Bovine Origin of Clonal Complex 17 of Streptococcus agalactiae. Appl. Environ. Microbiol. 73: 4668-4672 [Abstract] [Full Text]  
  • Nightingale, K. K., Lyles, K., Ayodele, M., Jalan, P., Nielsen, R., Wiedmann, M. (2006). Novel Method To Identify Source-Associated Phylogenetic Clustering Shows that Listeria monocytogenes Includes Niche-Adapted Clonal Groups with Distinct Ecological Preferences.. J. Clin. Microbiol. 44: 3742-3751 [Abstract] [Full Text]  
  • Ramaswamy, S. V., Ferrieri, P., Flores, A. E., Paoletti, L. C. (2006). Molecular characterization of nontypeable group B streptococcus.. J. Clin. Microbiol. 44: 2398-2403 [Abstract] [Full Text]  
  • Seifert, K. N., Adderson, E. E., Whiting, A. A., Bohnsack, J. F., Crowley, P. J., Brady, L. J. (2006). A unique serine-rich repeat protein (Srr-2) and novel surface antigen ({varepsilon}) associated with a virulent lineage of serotype III Streptococcus agalactiae.. Microbiology 152: 1029-1040 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»