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Journal of Clinical Microbiology, August 2005, p. 4046-4051, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4046-4051.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of the Roche LightCycler Strep B Assay for Detection of Group B Streptococcus from Vaginal and Rectal Swabs

James R. Uhl,¹ Emily A. Vetter,¹ Kristi L. Boldt,² Bruce W. Johnston,² Kirk D. Ramin,² Marla J. Adams,² Patricia Ferrieri,⁴ Udo Reischl,⁵ and Franklin R. Cockerill III^1,3*

Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology,¹ Department of Obstetrics and Gynecology,² Division of Infectious Diseases, Department of Internal Medicine, Mayo Clinic and Mayo Clinic College of Medicine, Rochester, Minnesota,³ Department of Laboratory Medicine and Pathology and Department of Pediatrics, University of Minnesota Medical School, Minneapolis, Minnesota,⁴ Institute of Medical Microbiology and Hygiene, University of Regensburg, Regensburg, Germany⁵

Received 29 October 2004/ Returned for modification 6 December 2004/ Accepted 10 May 2005

The results for a real-time PCR assay, using the LightCycler Strep B analyte-specific reagents (Roche Diagnostics Corporation, Indianapolis, Ind.), were compared to a direct plate method combined with a broth enrichment culture method for detection of group B streptococcus colonization in pregnant women. Two separate evaluations were conducted using two different automated nucleic extraction instruments, the MagNA Pure LC instrument (Roche Diagnostics Corporation) and the lower-capacity MagNA Pure Compact instrument (Roche Diagnostics Corporation). The sensitivities, specificities, and positive and negative predictive values for the different evaluation methods were as follow: for the LightCycler Strep B assay with MagNA Pure LC, 100, 97, 90, and 100%, respectively; for the LightCycler Strep B assay with MagNA Pure Compact, 92.5, 99, 97, and 97.5%, respectively. The LightCycler Strep B assay combined with either MagNA Pure LC or MagNA Pure Compact extraction is a suitable method for detecting group B streptococcus colonization in pregnant women. An advantage of the LightCycler assay over culture is the considerably reduced turnaround time for results.

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* Corresponding author. Mailing address: Mayo Clinic, 200 First Street SW, Hilton 470B, Rochester, MN 55905. Phone: (507) 284-2901. Fax: (507) 284-4272. E-mail: cockerill.franklin{at}mayo.edu.

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Journal of Clinical Microbiology, August 2005, p. 4046-4051, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4046-4051.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

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