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Journal of Clinical Microbiology, August 2005, p. 4186-4188, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4186-4188.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison between Sequence Analysis and a Line Probe Assay for Testing Genotypic Resistance of Human Immunodeficiency Virus Type 1 to Antiretroviral Drugs

S. García-Bujalance,1* C. Ladrón de Guevara,1 J. González-García,2 J. R. Arribas,2 and A. Gutiérrez1

Department of Microbiology and Parasitology,1 Department of Internal Medicine, La Paz University Hospital, Paseo de la Castellana 261, 28046 Madrid, Spain2

Received 17 June 2004/ Returned for modification 8 November 2004/ Accepted 22 April 2005

The purpose of this study was to compare a line probe assay (LiPA) with sequence analysis for the detection of mutations conferring resistance to nucleoside and non-nucleoside inhibitors in human immunodeficiency reverse transcriptase and protease inhibitors. The limitations for interpreting LiPA make it unacceptable for routine clinical practice.

* Corresponding author. Mailing address: Department of Microbiology and Parasitology, La Paz University Hospital, Paseo de la Castellana 261, 28046 Madrid, Spain. Phone: 34-91-7277248, x2452. Fax: 34-91-7277372. E-mail: sgarciab.hulp{at}salud.madrid.org.

Journal of Clinical Microbiology, August 2005, p. 4186-4188, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4186-4188.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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