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Journal of Clinical Microbiology, August 2005, p. 4249-4250, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4249-4250.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Stability of Human Immunodeficiency Virus Type 1 Proviral DNA in Whole-Blood Samples

Cheryl Jennings,1* Aleksandra Danilovic,1 Salvatore Scianna,1 Donald J. Brambilla,2 and James W. Bremer1

Rush Medical College, 1653 W. Congress Parkway, Chicago, Illinois 60612,1 New England Research Institutes, Inc., 9 Galen Street, Watertown, Massachusetts 024722

Received 3 February 2005/ Accepted 23 April 2005

The Roche Amplicor HIV-1 Test requires that whole blood be processed within four days of collection. However, this requirement may be too limiting for use in international settings. Thus, we demonstrate that blood may be processed up to 10 days after collection if maintained under ambient conditions (2 to 25°C).

* Corresponding author. Mailing address: Rush Medical College, Department of Immunology/Microbiology, 1653 W. Congress Parkway, Chicago, IL 60612. Phone: (312) 942-5954. Fax: (312) 942-6787. E-mail: cjenning{at}rush.edu.

Journal of Clinical Microbiology, August 2005, p. 4249-4250, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4249-4250.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mitchell, C., Jennings, C., Brambilla, D., Aldrovandi, G., Amedee, A. M., Beck, I., Bremer, J. W., Coombs, R., Decker, D., Fiscus, S., Fitzgibbon, J., Luzuriaga, K., Moye, J., Palumbo, P., Reichelderfer, P., Somasundaran, M., Stevens, W., Frenkel, L., for the Dried Blood Spot Working Group of the Infa, (2008). Diminished Human Immunodeficiency Virus Type 1 DNA Yield from Dried Blood Spots after Storage in a Humid Incubator at 37{degrees}C Compared to -20{degrees}C. J. Clin. Microbiol. 46: 2945-2949 [Abstract] [Full Text]  


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