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Journal of Clinical Microbiology, September 2005, p. 4426-4433, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4426-4433.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Detection of Antibodies against Adenovirus Protein IX, Fiber, and Hexon in Human Sera by Immunoblot Assay

Ulrike Bauer,1 Gisela Flunker,1 Kornelia Bruss,1 Knut Kallwellis,1 Herbert Liebermann,1 Tanja Luettich,2 Manfred Motz,2 and Werner Seidel1*

Friedrich Loeffler Institute of Medical Microbiology, University of Greifswald, Greifswald,1 Mikrogen GmbH, Munich-Martinsried, Germany2

Received 30 June 2005/ Returned for modification 20 February 2005/ Accepted 26 May 2005

The 51 serotypes of human adenoviruses (HAdVs) of the genus Mastadenovirus are classified into the six species HAdV-A to HAdV-F. For the detection of genus- and species-specific antibodies in human sera an immunoblot assay was developed. The recombinant long fiber of HAdV-41[F] (Ad41Fi) and the native hexon of HAdV-5[C] were used as genus-specific antigens. The recombinant capsid protein IX (pIX) of HAdV-2 (Ad2pIX[C]) and HAdV-41 (Ad41pIX[F]), the C-terminal pIX part of HAdV-3 (Ad3pIXC[B]), and the fiber knob of HAdV-8 (Ad8FiKn[D]) were evaluated as representative species-specific antigens. Hence, the pIX amino acid sequences of numerous serotypes of all HAdV species were compared, and the cross-reactivities of pIX antigens with rabbit hyperimmune sera among HAdV-A to -F were analyzed. In an epidemiological study, 667 human patient sera, not selected for viral infection, were screened for adenovirus seroprevalence. The genus-specific antibody prevalences directed against the Ad41Fi and HAdV-5 hexon were 82.8 and 98.8%, respectively. The species-specific antibody prevalence of 44.7% against Ad2pIX[C], 36.6% against Ad41pIX[F], 26.4% against Ad8FiKn[D], and 18% against Ad3pIXC[B] showed an age-dependent distribution and correlated well with the frequency of isolated serotypes of the respective species in earlier studies (except HAdV-D). In conclusion, the immunoblot assay using pIX, fiber, and hexon antigens represents a valuable and new serological tool for refined adenovirus diagnosis as shown in an epidemiological study.

* Corresponding author. Mailing address: Friedrich Loeffler Institute of Medical Microbiology, University of Greifswald, Lutherstrasse 6, D-17487 Greifswald, Germany. Phone: 49-3834-865567. Fax: 49-3834-865561. E-mail: seidel{at}uni-greifswald.de.

Journal of Clinical Microbiology, September 2005, p. 4426-4433, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4426-4433.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Appaiahgari, M. B., Pandey, R. M., Vrati, S. (2007). Seroprevalence of Neutralizing Antibodies to Adenovirus Type 5 among Children in India: Implications for Recombinant Adenovirus-Based Vaccines. CVI 14: 1053-1055 [Abstract] [Full Text]  


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