This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Barin, F.
Right arrow Articles by Costagliola, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barin, F.
Right arrow Articles by Costagliola, D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2005, p. 4441-4447, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4441-4447.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Development and Validation of an Immunoassay for Identification of Recent Human Immunodeficiency Virus Type 1 Infections and Its Use on Dried Serum Spots

Francis Barin,1* Laurence Meyer,2 Rémi Lancar,3 Christiane Deveau,2 Myriam Gharib,2 Anne Laporte,4 Jean-Claude Desenclos,4 and Dominique Costagliola3

Université François-Rabelais, EA3856 and Centre National de Référence du VIH, Tours,1 INSERM U569, Epidemiology Department, Hôpital Bicêtre AP-HP, Le Kremlin-Bicêtre,2 INSERM U720, Université Pierre et Marie Curie, Paris,3 Institut de Veille Sanitaire, Saint-Maurice, France4

Received 19 March 2005/ Returned for modification 12 May 2005/ Accepted 28 May 2005

The objective was to develop and to validate an immunossay to identify recent human immunodeficiency virus type 1 (HIV-1) infections that can be used on dried serum spots (DSS). A single, indirect enzyme-linked immunosorbent assay was developed to quantify antibodies toward four HIV-1 antigens: consensus peptides of the immunodominant epitope of gp41 (IDE), consensus V3 peptides, recombinant integrase, and recombinant p24. The parameters of the logistic regression used to classify the samples were estimated on a training sample (210 serum samples) using resampling techniques to get stable estimates and then applied to a validation sample (761 serum samples). The IDE and V3 peptides were the best able to discriminate between the antibodies present in serum from recently (≤6 months) infected individuals and those with long-lasting infection. Combined quantification of antibody binding to these two synthetic antigens allowed us to identify recent infections with an area under the receiver operating characteristic curve of 0.949 and a sensitivity of 88.3%, with a specificity of 97.6% in patients with long-term infection (but not AIDS) and 86.0% in patients suffering from AIDS with a threshold of 0.50 in the validation sample. This simple immunoassay can be used to identify recently HIV-1-infected patients. Its performance is compatible with its use in population-based studies including DSS.

* Corresponding author. Mailing address: Laboratoire de Virologie, Centre National de Référence du VIH, Centre Hospitalier Universitaire Bretonneau, 37044 Tours Cedex, France. Phone: 33 2 47 47 80 58. Fax: 33 2 47473610. E-mail: fbarin{at}med.univ-tours.fr.

Journal of Clinical Microbiology, September 2005, p. 4441-4447, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4441-4447.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Desbois, D., Roque-Afonso, A.-M., Lebraud, P., Dussaix, E. (2009). Use of Dried Serum Spots for Serological and Molecular Detection of Hepatitis A Virus. J. Clin. Microbiol. 47: 1536-1542 [Abstract] [Full Text]  
  • Monleau, M., Montavon, C., Laurent, C., Segondy, M., Montes, B., Delaporte, E., Boillot, F., Peeters, M. (2009). Evaluation of Different RNA Extraction Methods and Storage Conditions of Dried Plasma or Blood Spots for Human Immunodeficiency Virus Type 1 RNA Quantification and PCR Amplification for Drug Resistance Testing. J. Clin. Microbiol. 47: 1107-1118 [Abstract] [Full Text]  
  • Derache, A., Maiga, A.-I., Traore, O., Akonde, A., Cisse, M., Jarrousse, B., Koita, V., Diarra, B., Carcelain, G., Barin, F., Pizzocolo, C., Pizarro, L., Katlama, C., Calvez, V., Marcelin, A.-G. (2008). Evolution of genetic diversity and drug resistance mutations in HIV-1 among untreated patients from Mali between 2005 and 2006. J Antimicrob Chemother 62: 456-463 [Abstract] [Full Text]  
  • Loschen, S., Batzing-Feigenbaum, J., Poggensee, G., Cordes, C., Hintsche, B., Rausch, M., Dupke, S., Gohlke-Micknis, S., Rodig, J., Hamouda, O., Kucherer, C. (2008). Comparison of the Human Immunodeficiency Virus (HIV) Type 1-Specific Immunoglobulin G Capture Enzyme-Linked Immunosorbent Assay and the Avidity Index Method for Identification of Recent HIV Infections. J. Clin. Microbiol. 46: 341-345 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»