This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Page, B. T.
Right arrow Articles by Kurtzman, C. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Page, B. T.
Right arrow Articles by Kurtzman, C. P.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2005, p. 4507-4514, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4507-4514.2005

Rapid Identification of Candida Species and Other Clinically Important Yeast Species by Flow Cytometry{dagger}

Brent T. Page and Cletus P. Kurtzman*

Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois

Received 7 January 2005/ Returned for modification 23 February 2005/ Accepted 2 June 2005

Two rapid diagnostic assays, utilizing two different Luminex flow cytometry methods, were developed for identification of clinically important ascomycetous yeast species. Direct hybridization and allele-specific primer extension methods were both successful in establishing a DNA-based assay that can rapidly and accurately identify Candida albicans, Candida krusei, Candida parapsilosis, Candida glabrata, and Candida tropicalis as well as other clinical species. The direct hybridization assay was designed to identify a total of 19 ascomycetous yeast species, and the allele-specific primer extension assay was designed to identify a total of 34 species. Probes were validated against 438 strains representing 303 species. From culture to identification, the allele-specific primer extension method takes 8 h and the direct hybridization method takes less than 5 h to complete. These assays represent comprehensive, rapid tests that are well suited for the clinical laboratory.

* Corresponding author. Mailing address: Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 N. University St., Peoria, IL 61604. Phone: (309) 681-6561. Fax: (309) 681-6672. E-mail: Kurtzman{at}ncaur.usda.gov.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, September 2005, p. 4507-4514, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4507-4514.2005




This article has been cited by other articles:

  • Oechsler, R. A., Feilmeier, M. R., Ledee, D. R., Miller, D., Diaz, M. R., Fini, M. E., Fell, J. W., Alfonso, E. C. (2009). Utility of Molecular Sequence Analysis of the ITS rRNA Region for Identification of Fusarium spp. from Ocular Sources. IOVS 50: 2230-2236 [Abstract] [Full Text]  
  • Landlinger, C., Preuner, S., Willinger, B., Haberpursch, B., Racil, Z., Mayer, J., Lion, T. (2009). Species-Specific Identification of a Wide Range of Clinically Relevant Fungal Pathogens by Use of Luminex xMAP Technology. J. Clin. Microbiol. 47: 1063-1073 [Abstract] [Full Text]  
  • Huang, C.-T., Wong, W.-W., Li, L.-H., Chiang, C.-C., Chen, B.-D., Li, S.-Y. (2008). Genotyping of Chlamydia trachomatis by Microsphere Suspension Array. J. Clin. Microbiol. 46: 1126-1128 [Abstract] [Full Text]  
  • Peterson, S. W. (2008). Phylogenetic analysis of Aspergillus species using DNA sequences from four loci. Mycologia 100: 205-226 [Abstract] [Full Text]  
  • Lin, Y.-C., Sheng, W.-H., Chang, S.-C., Wang, J.-T., Chen, Y.-C., Wu, R.-J., Hsia, K.-C., Li, S.-Y. (2008). Application of a Microsphere-Based Array for Rapid Identification of Acinetobacter spp. with Distinct Antimicrobial Susceptibilities. J. Clin. Microbiol. 46: 612-617 [Abstract] [Full Text]  
  • Bovers, M., Diaz, M. R., Hagen, F., Spanjaard, L., Duim, B., Visser, C. E., Hoogveld, H. L., Scharringa, J., Hoepelman, I. M., Fell, J. W., Boekhout, T. (2007). Identification of Genotypically Diverse Cryptococcus neoformans and Cryptococcus gattii Isolates by Luminex xMAP Technology. J. Clin. Microbiol. 45: 1874-1883 [Abstract] [Full Text]  
  • Wise, M. G., Healy, M., Reece, K., Smith, R., Walton, D., Dutch, W., Renwick, A., Huong, J., Young, S., Tarrand, J., Kontoyiannis, D. P. (2007). Species identification and strain differentiation of clinical Candida isolates using the DiversiLab system of automated repetitive sequence-based PCR. J Med Microbiol 56: 778-787 [Abstract] [Full Text]  
  • Diaz, M. R., Boekhout, T., Theelen, B., Bovers, M., Cabanes, F. J., Fell, J. W. (2006). Microcoding and flow cytometry as a high-throughput fungal identification system for Malassezia species.. J Med Microbiol 55: 1197-1209 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»