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Journal of Clinical Microbiology, September 2005, p. 4654-4658, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4654-4658.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Search for Localized Dysbiosis in Crohn's Disease Ulcerations by Temporal Temperature Gradient Gel Electrophoresis of 16S rRNA

Philippe Seksik,1,2,{dagger} Patricia Lepage,1,{dagger} Marie-France de la Cochetière,3 Arnaud Bourreille,4 Malène Sutren,1 Jean-Paul Galmiche,4 Joël Doré,1 and Philippe Marteau2*

INRA, CR de Jouy-en-Josas, 78352 Jouy-en-Josas, France,1 Département d'Hépato-Gastroentérologie, Hôpital Européen Georges Pompidou, AP-HP, Paris, France,2 INSERM-U539, Nantes, France,3 Département d'Hépato-Gastroentérologie, Hôpital de l'Hotel Dieu, Nantes, France4

Received 23 March 2005/ Returned for modification 17 May 2005/ Accepted 1 June 2005

The mucosa-associated microbiota lining the gut epithelium might play a central role in the activation and/or perpetuation of mucosal inflammation in Crohn's disease (CD). We sought for localized dysbiosis by comparing the biodiversity and composition of the microbiotas in ulcerated and nonulcerated mucosal samples from patients with CD. Biopsy samples (n = 75) of ulcerated and adjacent nonulcerated mucosa were collected during colonoscopy from 15 patients, from the ileum, right colon, left colon, and rectum. Temporal temperature gradient gel electrophoresis (TTGE) of bacterial 16S rRNAs was used to evaluate the dominant bacterial species. TTGE profiles were compared using software that calculates similarity percentages. For a given patient, average similarity indexes between ulcerated and nonulcerated mucosal TTGE profiles ranged from 95.2% ± 4.2% to 97.9% ± 1.7% (means ± standard deviations) for the different segments. The mean values did not differ significantly. Average interindividual similarity indexes for a given segment among the different patients ranged from 33.6% ± 15.5% to 42.0% ± 25.6%. In CD, the dominant microbiotas do not differ qualitatively between ulcerated and nonulcerated mucosae. Biodiversity remains high in ulcerated mucosa. This argues against a pathogenic role of localized qualitative dysbiosis in CD-associated ulceration.

* Corresponding author. Mailing address: Service de Gastro-entérologie, Hôpital Européen Georges Pompidou, 20 rue Leblanc, 75908 Paris cedex 15, France. Phone: 33 1 5609 3562. Fax: 33 1 5609 3529. E-mail: philippe.marteau{at}hop.egp.ap-hop-paris.fr.

{dagger} These authors contributed equally to this study.

Journal of Clinical Microbiology, September 2005, p. 4654-4658, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4654-4658.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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