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Journal of Clinical Microbiology, September 2005, p. 4696-4703, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4696-4703.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Properties of Novel International Drug-Resistant Pneumococcal Clones Identified in Day-Care Centers of Lisbon, Portugal

Natacha G. Sousa,1 Raquel Sá-Leão,1 M. Inês Crisóstomo,1,2 Carla Simas,1 Sónia Nunes,1 Nelson Frazão,1 João A. Carriço,3 Rosario Mato,1 Ilda Santos-Sanches,1,4 and Hermínia de Lencastre1,2*

Laboratório de Genética Molecular, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal,1 Laboratory of Microbiology, The Rockefeller University, New York, New York,2 Grupo de Biomatemática, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal,3 Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Monte da Caparica, Portugal4

Received 12 January 2005/ Returned for modification 15 March 2005/ Accepted 16 June 2005

In this study, 61 drug-resistant Streptococcus pneumoniae strains were characterized by multilocus sequence typing (MLST). These strains were representatives of 26 major clones (defined using pulsed-field gel electrophoresis) accounting for 93% of the 1,285 drug-resistant Streptococcus pneumoniae isolates recovered from the nasopharynges of healthy children attending day-care centers in Lisbon during 2001 to 2003. Using MLST, 13 of the 26 clones were found to be identical or closely related to 11 Pneumococcal Molecular Epidemiology Network (PMEN) clones, 4 clones were found to be unique as there were no identical or highly related allelic profiles deposited in the MLST database, and the remaining 9 clones had sequence types that matched or differed at a single or double locus from allelic profiles available in the MLST database. These nine clones were of serotypes 33F, 10A, 19A, 19F, 6A, 20, 24F, and 3, one was nontypeable, and, by MLST, they were found to be identical or highly related to isolates from disease origin that were dispersed internationally. Since the majority of these clones had serotypes that are not included in the 7-valent conjugate pneumococcal vaccine, monitoring of these clones is important for surveying their possible spread in the future. We propose the inclusion of these novel international clones in the PMEN.

* Corresponding author. Mailing address: The Rockefeller University, 1230 York Avenue, New York, NY 10021. Phone: (212) 327-8278. Fax: (212) 327-8688. E-mail: lencash{at}rockefeller.edu.

Journal of Clinical Microbiology, September 2005, p. 4696-4703, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4696-4703.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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