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Journal of Clinical Microbiology, September 2005, p. 4731-4736, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4731-4736.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Multilocus Sequence Typing of Staphylococcus aureus Isolated from High-Somatic-Cell-Count Cows and the Environment of an Organic Dairy Farm in the United Kingdom

E. M. Smith, L. E. Green,^* G. F. Medley, H. E. Bird, and C. G. Dowson

Department of Biological Sciences, The University of Warwick, Coventry CV4 7AL, United Kingdom

Received 21 December 2004/ Returned for modification 15 April 2005/ Accepted 30 May 2005

An outbreak of Staphylococcus aureus intramammary infections on an organic dairy farm was monitored for 10 months. Environmental and milk samples were collected from a total of 26 cows and a group of 21 purchased heifers about to be introduced into the milking herd. There was variation in the rate of isolation of S. aureus (9.5 to 43.8%) from individual mammary quarters, although no S. aureus isolates were detected in the milk samples collected from the heifers. One hundred ninety-one S. aureus isolates were detected from cow milk samples (n = 182), milking machine clusters (n = 4), farm personnel (n = 4), and the environment (n = 1). Multilocus sequence typing (MLST) had a typeability of 100% when it was applied to the 191 isolates. Among the 191 isolates there was limited strain diversity, with seven sequence types (STs) dominated by two strains with closely related STs that differed at a single locus. Within individual mammary quarters there were naturally occurring dual infections, although this was identified in only 0.4% of milk samples. Different strains were associated with variable persistence within quarters. MLST is clearly a very suitable tool for the differentiation and analysis of S. aureus populations detected on dairy cattle farms.

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* Corresponding author. Mailing address: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom. Phone: 44 (0) 24 7652 3797. Fax: 44 (0) 24 7652 4619. E-mail: laura.green{at}warwick.ac.uk.

Present address: Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI 53226.

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Journal of Clinical Microbiology, September 2005, p. 4731-4736, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4731-4736.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

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