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Journal of Clinical Microbiology, September 2005, p. 4823-4825, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4823-4825.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

PCR-Based Diagnosis of Neonatal Staphylococcal Bacteremias

Imad R. Makhoul,1 Tatiana Smolkin,1 Polo Sujov,1 Imad Kassis,2 Ada Tamir,3 Raia Shalginov,4 and Hannah Sprecher4*

Departments of Neonatology,1 Infectious Diseases,2 Epidemiology,3 Laboratory of Clinical Microbiology, Meyer Childrens Hospital—Rambam Medical Center, and the Bruce Rappaport Faculty of Medicine, Technion—Israel Institute of Technology, Haifa, Israel4

Received 15 March 2005/ Returned for modification 25 April 2005/ Accepted 16 June 2005

We compared PCR with blood cultures in the diagnosis of neonatal staphylococcal sepsis. Significant association was observed between PCR-based and culture-based diagnosis of staphylococcal bacteremia. Positive and negative predictive values for PCR were 100% and 98%, respectively. These data indicate that PCR may serve as a useful adjunct for the rapid diagnosis of staphylococcal sepsis.

* Corresponding author. Mailing address: Department of Microbiology, Rambam Medical Center, Bat-Galim, Haifa, 31096, Israel. Phone: 972-4-8542-219. Fax: 972-4-8543-430. E-mail: h_sprecher{at}rambam.health.gov.il.

Journal of Clinical Microbiology, September 2005, p. 4823-4825, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4823-4825.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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