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Journal of Clinical Microbiology, September 2005, p. 4840-4843, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4840-4843.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Human Herpesvirus 8 Serological Markers and Viral Load in Patients with AIDS-Associated Kaposi's Sarcoma in Central African Republic

Renan Duprez,1 Eric Kassa-Kelembho,2 Sabine Plancoulaine,3 Josette Brière,4 Mireille Fossi,2 Léon Kobangue,5 Pierre Minsart,6 Michel Huerre,7 and Antoine Gessain1*

Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Département Ecosystèmes et Epidémiologie des Maladies Infectieuses, Institut Pasteur, Paris, France,1 Institut Pasteur de Bangui, Bangui, Central African Republic,2 Laboratoire de Génétique Humaine des Maladies Infectieuses, INSERM U.550, Faculté de Médecine Necker, Paris, France,3 Laboratoire d'Anatomo-pathologie, Hopital Saint-Louis, Paris, France,4 Centre National Hospitalier Universitaire de Bangui, Bangui, Central African Republic,5 Hopital Communautaire de Bangui, Bangui, Central African Republic,6 Unité de Recherche et d'Expertise Histotechnologie et Pathologie, Institut Pasteur, Paris, France7

Received 9 March 2005/ Returned for modification 18 April 2005/ Accepted 13 June 2005

Epidemic Kaposi's sarcoma (KS) is one of the most frequent types of cancer in several African countries; however, very few data are available on human herpesvirus 8 (HHV-8) markers in KS patients from Central Africa. In a series of 36 AIDS-KS cases from Central African Republic, we showed, using a real-time PCR quantitative assay, the high frequency (82%) of detectable HHV-8 DNA in peripheral blood mononuclear cells (PBMCs). We also found that the level of antibodies directed against lytic or latent HHV-8 antigens is not correlated to the amount of HHV-8 viral load in the PBMCs, and finally, we demonstrated a much higher viral load in tumoral skin lesions (6.07 log copies/µg DNA) than in unaffected skin (2.93 log copies/µg DNA) or in PBMCs (2.55 log copies/µg DNA).

* Corresponding author. Mailing address: Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Département Ecosytèmes et Epidémiologie des Maladies Infectieuses. Batiment Lwoff., Institut Pasteur, 25-28 rue du Dr. Roux, 75724, Paris, Cedex 15, France. Phone: 33 (0)1 45 68 89 37. Fax: 33 (0)1 40 61 34 65. E-mail: agessain{at}pasteur.fr.

Journal of Clinical Microbiology, September 2005, p. 4840-4843, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4840-4843.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Adang, L. A., Parsons, C. H., Kedes, D. H. (2006). Asynchronous Progression through the Lytic Cascade and Variations in Intracellular Viral Loads Revealed by High-Throughput Single-Cell Analysis of Kaposi's Sarcoma-Associated Herpesvirus Infection.. J. Virol. 80: 10073-10082 [Abstract] [Full Text]  


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