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Journal of Clinical Microbiology, September 2005, p. 4855-4857, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4855-4857.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Rapid Identification of Staphylococcus aureus in Blood Cultures by a Combination of Fluorescence In Situ Hybridization Using Peptide Nucleic Acid Probes and Flow Cytometry

Hanna Hartmann,1 Henrik Stender,2 Andrea Schäfer,1 Ingo B. Autenrieth,1 and Volkhard A. J. Kempf1*

Institut für Medizinische Mikrobiologie und Hygiene, Eberhard-Karls-Universität Tübingen, Elfriede-Aulhorn Str. 6, 72076 Tübingen, Germany,1 AdvanDx Inc., 25K Olympia Ave., Woburn, Massachusetts 018012

Received 20 April 2005/ Returned for modification 31 May 2005/ Accepted 2 June 2005

Fluorescence in situ hybridization (FISH) using peptide nucleic acid probes (PNAs) allows the identification of Staphylococcus aureus from human blood culture samples. We present data revealing that the combination of PNA FISH and flow cytometry is a possible approach for the noncultural identification of staphylococci in blood cultures.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie und Hygiene, Elfriede-Aulhorn-Str. 6, D-72076 Tübingen, Germany. Phone: 49-7071-2981526. Fax: 49-7071-295440. E-mail: volkhard.kempf{at}med.uni-tuebingen.de.

Journal of Clinical Microbiology, September 2005, p. 4855-4857, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4855-4857.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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