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Journal of Clinical Microbiology, September 2005, p. 4880-4884, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4880-4884.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Direct Application of the INNO-LiPA Rif.TB Line-Probe Assay for Rapid Identification of Mycobacterium tuberculosis Complex Strains and Detection of Rifampin Resistance in 360 Smear-Positive Respiratory Specimens from an Area of High Incidence of Multidrug-Resistant Tuberculosis

Miguel Viveiros,¹ Clara Leandro,¹ Liliana Rodrigues,¹ Josefina Almeida,¹ Rosário Bettencourt,¹ Isabel Couto,^1,2 Lurdes Carrilho,³ José Diogo,⁴ Ana Fonseca,⁵ Luís Lito,⁶ João Lopes,⁷ Teresa Pacheco,⁸ Mariana Pessanha,⁹ Judite Quirim,¹⁰ Luísa Sancho,¹¹ Max Salfinger,¹² and Leonard Amaral^1*

Unidade de Micobacterias, UPMM, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisboa, Portugal,¹ Centro de Recursos Microbiológicos (CREM), Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Lisboa, Portugal,² Laboratorio de Micobacterias, Hospital Pulido Valente, Lisboa, Portugal,³ Laboratorio de Microbiologia, Hospital Garcia da Orta, Lisboa, Portugal,⁴ Laboratorio de Microbiologia, Hospital Condes de Castro Guimarães, Cascais, Portugal,⁵ Laboratorio de Microbiologia, Hospital de Santa Maria, Lisboa, Portugal,⁶ Laboratorio de Microbiologia, Hospital de Nossa Senhora do Rosário, Barreiro, Portugal,⁷ Laboratorio de Microbiologia, Hospital Egas Moniz, Lisboa, Portugal,⁸ Laboratorio de Microbiologia, Hospital São Francisco Xavier, Lisboa, Portugal,⁹ Laboratorio de Microbiologia, Hospital de São Bernardo, Setúbal, Portugal,¹⁰ Laboratorio de Microbiologia, Hospital Fernando da Fonseca, Amadora-Sintra, Portugal,¹¹ Clinical Mycobacteriology Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York 12208,¹²

Received 11 June 2005/ Accepted 15 June 2005

The INNO-LiPA Rif.TB assay for the identification of Mycobacterium tuberculosis complex strains and the detection of rifampin (RIF) resistance has been evaluated with 360 smear-positive respiratory specimens from an area of high incidence of multidrug-resistant tuberculosis (MDR-TB). The sensitivity when compared to conventional identification/culture methods was 82.2%, and the specificity was 66.7%; the sensitivity and specificity were 100.0% and 96.9%, respectively, for the detection of RIF resistance. This assay has the potential to provide rapid information that is essential for the effective management of MDR-TB.

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* Corresponding author. Mailing address: Unidade de Micobacterias do Instituto de Higiene e Medicina Tropical, Rua Junqueira 96, 1349-008 Lisboa, Portugal. Phone: 351 21 3652653. Fax: 351 21 3632105. E-mail: lamaral{at}ihmt.unl.pt.

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Journal of Clinical Microbiology, September 2005, p. 4880-4884, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4880-4884.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

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