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Journal of Clinical Microbiology, January 2006, p. 35-41, Vol. 44, No. 1
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.1.35-41.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Multilocus Sequence Typing of Porphyromonas gingivalis Strains from Different Geographic Origins

Morten Enersen,1,3* Ingar Olsen,1 Arie J. van Winkelhoff,2 and Dominique A. Caugant1,3

Institute of Oral Biology, Dental Faculty, University of Oslo, 0316 Oslo, Norway,1 Department of Oral Microbiology, Academic Center for Dentistry Amsterdam, Amsterdam, The Netherlands,2 Department of Airborne Infections, Norwegian Institute of Public Health, 0403 Oslo, Norway3

Received 4 August 2005/ Returned for modification 17 September 2005/ Accepted 9 October 2005

Porphyromonas gingivalis is an important periodontal pathogen that can be isolated from both active and inactive periodontal lesions. Apparently, differences in virulence between P. gingivalis strains exist, but the mechanisms underlying these differences are not yet fully understood. To obtain more information about pathogenicity and virulence of P. gingivalis, it is relevant to assess the genetic population structure of the species and to examine the occurrence of putative virulence factors against the genetic background. Presently, multilocus sequence typing (MLST) is the best method for analyzing bacterial population structures. Forty P. gingivalis strains from worldwide sources were analyzed by MLST. Internal 310- to 420-bp DNA fragments of the eight ubiquitous chromosomal genes, ftsQ, hagB, gdpxJ, pepO, mcmA, recA, pga, and nah, were amplified by PCR and then sequenced. The number of alleles at individual loci ranged from 2 to 19, and a total of 33 allelic profiles, or sequence types (STs), were identified. Nucleotide variation between alleles was located at one or a few sites. Identical or similar STs were found in isolates from different geographic regions. Our results showed signs of a clonal population structure with a level of recombination not as high as that previously suggested for the species. We also found that P. gingivalis isolates from individual patients were genetically heterogeneous.

* Corresponding author. Mailing address: Department of Airborne Infections, Norwegian Institute of Public Health, P.O. Box 4404 Nydalen, NO-0403 Oslo, Norway. Phone: 47 22840353. Fax: 47 840301. E-mail: morteene{at}odont.uio.no.

Journal of Clinical Microbiology, January 2006, p. 35-41, Vol. 44, No. 1
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.1.35-41.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Do, T., Jolley, K. A., Maiden, M. C. J., Gilbert, S. C., Clark, D., Wade, W. G., Beighton, D. (2009). Population structure of Streptococcus oralis. Microbiology 155: 2593-2602 [Abstract] [Full Text]  
  • Enersen, M., Olsen, I., Caugant, D. A. (2008). Genetic Diversity of Porphyromonas gingivalis Isolates Recovered from Single "Refractory" Periodontitis Sites. Appl. Environ. Microbiol. 74: 5817-5821 [Abstract] [Full Text]  
  • Enersen, M., Olsen, I., Kvalheim, O., Caugant, D. A. (2008). fimA Genotypes and Multilocus Sequence Types of Porphyromonas gingivalis from Patients with Periodontitis. J. Clin. Microbiol. 46: 31-42 [Abstract] [Full Text]  
  • Tribble, G. D., Lamont, G. J., Progulske-Fox, A., Lamont, R. J. (2007). Conjugal Transfer of Chromosomal DNA Contributes to Genetic Variation in the Oral Pathogen Porphyromonas gingivalis. J. Bacteriol. 189: 6382-6388 [Abstract] [Full Text]  


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