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Journal of Clinical Microbiology, October 2006, p. 3659-3664, Vol. 44, No. 10
0095-1137/06/$08.00+0     doi:10.1128/JCM.01054-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Performance of the Genotype MTBDR Line Probe Assay for Detection of Resistance to Rifampin and Isoniazid in Strains of Mycobacterium tuberculosis with Low- and High-Level Resistance

Florence Brossier, Nicolas Veziris, Chantal Truffot-Pernot, Vincent Jarlier, and Wladimir Sougakoff*

National Reference Center for Mycobacteria, Department of Bacteriology-Hygiene, Faculté de Médecine Pierre & Marie Curie (Paris VI) site Pitié-Salpêtrière, Paris, France

Received 19 May 2006/ Returned for modification 3 July 2006/ Accepted 6 August 2006

We assessed the performance of the Genotype MTBDR line probe assay that offers the simultaneous identification of Mycobacterium tuberculosis and its resistance to rifampin (RIF) and isoniazid (INH) by detecting the most commonly found mutations in the rpoB and katG genes. One hundred thirteen M. tuberculosis isolates were tested. The nucleotide sequences of the katG and inhA genes and the mabA-inhA promoter region were also determined. The MTBDR assay detected 100% and 67% (n = 64) of the strains resistant to RIF and INH, respectively. Among the latter, 62 strains carried a Ser315Thr mutation in katG, 59 of them displaying a high level of resistance to INH. Two strains with a low level of INH resistance had a Ser315Asn mutation. No mutation was found by the MTBDR assay for 31 INH-resistant strains (33%), of which 24 showed a low level of resistance. By DNA sequencing, we found among them various mutations in the KatG protein for 7 strains, a C->T mutation in position –15 of the mabA-inhA promoter in 17 strains, and a Ser94Ala mutation in InhA for 7 strains. In conclusion, the MTBDR assay, which fits easily in the workflow of a routine laboratory, enabled the detection of 100% of the RIF-resistant strains and 89% of the INH-resistant strains with a high level of resistance but only 17% of the strains characterized by a low level of INH resistance, indicating that the test can be used as a rapid method to detect in the same experiment the rifampin-resistant and the high-level isoniazid-resistant strains of M. tuberculosis.

* Corresponding author. Mailing address: Laboratoire de Bactériologie-Hygiène, Faculté de Médecine Pitié-Salpêtrière, 91 boulevard de l'Hôpital, F-75634 Paris cedex 13, France. Phone: 33 1 40 77 97 46. Fax: 33 1 45 82 75 77. E-mail: sougakof{at}chups.jussieu.fr.

Journal of Clinical Microbiology, October 2006, p. 3659-3664, Vol. 44, No. 10
0095-1137/06/$08.00+0     doi:10.1128/JCM.01054-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ho, Y. M., Sun, Y.-J., Wong, S.-Y., Lee, A. S. G. (2009). Contribution of dfrA and inhA Mutations to the Detection of Isoniazid-Resistant Mycobacterium tuberculosis Isolates. Antimicrob. Agents Chemother. 53: 4010-4012 [Abstract] [Full Text]  
  • Lacoma, A., Garcia-Sierra, N., Prat, C., Ruiz-Manzano, J., Haba, L., Roses, S., Maldonado, J., Dominguez, J. (2008). GenoType MTBDRplus Assay for Molecular Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis Strains and Clinical Samples. J. Clin. Microbiol. 46: 3660-3667 [Abstract] [Full Text]  
  • Khue, P. M., Truffot-Pernot, C., Texier-Maugein, J., Jarlier, V., Robert, J., on behalf of the AZAY-Mycobacteria Study Group, (2007). A 10-year prospective surveillance of Mycobacterium tuberculosis drug resistance in France 1995 2004. Eur Respir J 30: 937-944 [Abstract] [Full Text]  


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