This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mueller, M.
Right arrow Articles by Hartung, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mueller, M.
Right arrow Articles by Hartung, T.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, October 2006, p. 3778-3780, Vol. 44, No. 10
0095-1137/06/$08.00+0     doi:10.1128/JCM.00371-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Identification of Borrelia burgdorferi Ribosomal Protein L25 by the Phage Surface Display Method and Evaluation of the Protein's Value for Serodiagnosis

Markus Mueller,1,{dagger} Sebastian Bunk,1,{dagger} Isabel Diterich,1 Michael Weichel,2 Carolin Rauter,1 Dieter Hassler,3 Corinna Hermann,1 Reto Crameri,2 and Thomas Hartung1,4*

University of Konstanz, Biochemical Pharmacology, Konstanz, Germany,1 Swiss Institute of Allergy and Asthma Research, Daros, Switzerland,2 Untere Hofstaat 3, Kraichtal, Germany,3 ECVAM, EU Joint Research Centre, IHCP, Ispra, Italy4

Received 20 February 2006/ Returned for modification 19 April 2006/ Accepted 14 July 2006

The phage surface display technique was used to identify Borrelia burgdorferi antigens. By affinity selection with immunoglobulin G from pooled sera of six Lyme borreliosis (LB) patients, the ribosomal protein L25 was identified. The diagnostic value of L25 was investigated by an enzyme-linked immunosorbent assay, using sera from 80 LB patients and 75 controls, and the use of the protein resulted in a specificity of 99% and a 23% sensitivity, which qualify L25 as a useful antigen when combined with others.

* Corresponding author. Mailing address: University of Konstanz, Biochemical Pharmacology, 78457 Konstanz, Germany. Phone: 49-7531-884116. Fax: 49-7531-884117. E-mail: Thomas.Hartung{at}uni-konstanz.de.

{dagger} M.M. and S.B. contributed equally to this study.

Journal of Clinical Microbiology, October 2006, p. 3778-3780, Vol. 44, No. 10
0095-1137/06/$08.00+0     doi:10.1128/JCM.00371-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Coleman, A. S., Pal, U. (2009). BBK07, a Dominant In Vivo Antigen of Borrelia burgdorferi, Is a Potential Marker for Serodiagnosis of Lyme Disease. CVI 16: 1569-1575 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»