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Journal of Clinical Microbiology, October 2006, p. 3784-3786, Vol. 44, No. 10
0095-1137/06/$08.00+0     doi:10.1128/JCM.01373-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Detection of Norovirus Antigens from Recombinant Virus-Like Particles and Stool Samples by a Commercial Norovirus Enzyme-Linked Immunosorbent Assay Kit

Shoko Okitsu-Negishi,1* Michio Okame,1 Yuko Shimizu,1 Tung Gia Phan,1 Takeshi Tomaru,2 Shigenori Kamijo,2 Takashi Sato,2 Fumihiro Yagyu,1 Werner E. G. Müller,3 and Hiroshi Ushijima1

Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan,1 Nippon Becton Dickinson Company, Tokyo, Japan,2 Institüt fur Physiologische Chemie, Abteilung Angewandte Molekularbilogie, Johannes Gutenberg-Universitat, Mainz, Germany3

Received 4 July 2006/ Accepted 11 July 2006

The commercial norovirus enzyme-linked immunosorbent assay kit was evaluated for its reactivity to recombinant virus-like particles and the detection of natural viruses from stool samples of Japanese infants and children with sporadic acute gastroenteritis compared to reverse transcription-PCR. The kit had a sensitivity of 76.3% and a specificity of 94.9%. Our results clearly indicated that the kit allows the detection of the most prevalent genotype, GII/4. In order to increase the sensitivity of the kit, the reactivity with norovirus of GII/3 and GII/6 genotypes needs to be improved.

* Corresponding author. Mailing address: Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033, Japan. Phone: 81-3-5841-3590. Fax: 81-3-5841-3629. E-mail: mshoko{at}mail.ecc.u-tokyo.ac.jp.

Journal of Clinical Microbiology, October 2006, p. 3784-3786, Vol. 44, No. 10
0095-1137/06/$08.00+0     doi:10.1128/JCM.01373-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Shiota, T., Okame, M., Takanashi, S., Khamrin, P., Takagi, M., Satou, K., Masuoka, Y., Yagyu, F., Shimizu, Y., Kohno, H., Mizuguchi, M., Okitsu, S., Ushijima, H. (2007). Characterization of a Broadly Reactive Monoclonal Antibody against Norovirus Genogroups I and II: Recognition of a Novel Conformational Epitope. J. Virol. 81: 12298-12306 [Abstract] [Full Text]  


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