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Journal of Clinical Microbiology, October 2006, p. 3804-3807, Vol. 44, No. 10
0095-1137/06/$08.00+0     doi:10.1128/JCM.00395-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Comparison of PCR-Based Methods for Typing Staphylococcus aureus Isolates

Artur Sabat,1,{dagger} Natalia Malachowa,2,{dagger} Jacek Miedzobrodzki,2 and Waleria Hryniewicz1*

National Institute of Public Health, 00-725 Warsaw, Poland,1 Department of Microbiology, Faculty of Biotechnology, Jagiellonian University, 30-387 Cracow, Poland2

Received 22 February 2006/ Returned for modification 26 May 2006/ Accepted 7 July 2006

In this study, we compared the potentials of (i) a multiplex PCR-based multilocus variable-number tandem repeat (VNTR) assay; (ii) a triplex PCR coamplifying fragments of spa, coa, and the hypervariable region adjacent to the mecA gene; (iii) restriction profile analysis of the STAR repetitive element; (iv) randomly amplified polymorphic DNA analysis; (v) inter-IS256 PCR; and (vi) rep-MP3 PCR. Multilocus VNTR typing and triplex PCR (coa, spa, and hypervariable region) approaches showed excellent reproducibility and high discriminatory power; however, only multilocus VNTR typing could distinguish all pulsed-field gel electrophoresis and spa types. Multilocus VNTR typing appears to be the most useful PCR-based method for the rapid genotyping of Staphylococcus aureus strains.

* Corresponding author. Mailing address: National Institute of Public Health, 30/34 Chelmska St., 00-725 Warsaw, Poland. Phone: 48 22 841 33 67. Fax: 48 22 841 29 49. E-mail: waleria{at}cls.edu.pl.

{dagger} These authors contributed equally to this work.

Journal of Clinical Microbiology, October 2006, p. 3804-3807, Vol. 44, No. 10
0095-1137/06/$08.00+0     doi:10.1128/JCM.00395-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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