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Journal of Clinical Microbiology, October 2006, p. 3826-3829, Vol. 44, No. 10
0095-1137/06/$08.00+0     doi:10.1128/JCM.00225-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Evaluation of Molecular-Beacon, TaqMan, and Fluorescence Resonance Energy Transfer Probes for Detection of Antibiotic Resistance-Conferring Single Nucleotide Polymorphisms in Mixed Mycobacterium tuberculosis DNA Extracts

Hasan Yesilkaya,1 Francesca Meacci,2 Stefan Niemann,3 Doris Hillemann,3 Sabine Rüsch-Gerdes,3 LONG DRUG Study Group,{dagger} Michael R. Barer,1 Peter W. Andrew,1* and Marco R. Oggioni2

Department of Infection, Immunity and Inflammation, University of Leicester, Leicester LE1 9HN, United Kingdom,1 Laboratorio di Microbiologia Molecolare e Biotecnologia, Dipartimento di Biologia Molecolare, Universit à di Siena Policlinico Le Scotte (lotto 5, piano 1), 53100 Siena, Italy,2 National Reference Center for Mycobacteria, Forschungszentrum, Borstel, Germany3

Received 1 February 2006/ Returned for modification 5 April 2006/ Accepted 5 June 2006

The ability of fluorescence resonance energy transfer, molecular-beacon, and TaqMan probes to detect single nucleotide polymorphism (SNP) in the presence of a wild-type allele was evaluated using drug resistance-conferring SNPs in mixed Mycobacterium tuberculosis DNA. It was found that both the absolute quantity and the ratio of alleles determine the detection sensitivity of the probe systems.

* Corresponding author. Mailing address: Department of Infection, Immunity and Inflammation, University of Leicester, Leicester LE1 9HN, United Kingdom. Phone: (44) 116 2522941. Fax: (44) 116 2525030. E-mail: pwa{at}le.ac.uk.

{dagger} The LONG DRUG study group is composed of Marco R. Oggioni and Francesca Meacci, C Trappetti Università di Siena; Francesco Checchi and Maryline Bonnet, Epicentre Paris; Graziella Orefici, Manuela Pardini, and Lanfranco Fattorini, Istituto Superiore di Sanità Roma; Peter Andrew, Mike Barer, and Hasan Yesilkaya, University of Leicester; Heinz Rinder, University of München; Sabine Rüsch-Gerdes and Stefan Niemann, Research Centre Borstel; Germano Orrù, Università di Cagliari; Francis Varaine, Médecins Sans Frontières Paris; and Thierry Jarosz, 3Es Paris.

Journal of Clinical Microbiology, October 2006, p. 3826-3829, Vol. 44, No. 10
0095-1137/06/$08.00+0     doi:10.1128/JCM.00225-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Scott, J. C., Koylass, M. S., Stubberfield, M. R., Whatmore, A. M. (2007). Multiplex Assay Based on Single-Nucleotide Polymorphisms for Rapid Identification of Brucella Isolates at the Species Level. Appl. Environ. Microbiol. 73: 7331-7337 [Abstract] [Full Text]  


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