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Journal of Clinical Microbiology, November 2006, p. 4101-4112, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.01196-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Molecular Characterization of Novel G5 Bovine Rotavirus Strains

Sung-Hee Park,1 Linda J. Saif,2 Cheol Jeong,1 Guem-Ki Lim,1 Sang-Ik Park,1 Ha-Hyun Kim,1 Su-Jin Park,1 You-Jung Kim,1 Jae-Ho Jeong,1 Mun-Il Kang,1 and Kyoung-Oh Cho1*

Bio-therapy Human Resources Center, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, South Korea,1 Food Animal Health Research Program, Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio 446912

Received 10 June 2006/ Accepted 15 August 2006

Group A rotaviruses are a major cause of acute gastroenteritis in young children as well as many domestic animals. The rotavirus genome is composed of 11 segments of double-stranded RNA and can undergo genetic reassortment during mixed infections, leading to progeny viruses with novel or atypical phenotypes. The aim of this study was to determine if the bovine group A rotavirus strains KJ44 and KJ75, isolated from clinically infected calves, share genetic features with viruses obtained from heterologous species. All 11 genes sequences of the KJ44 and KJ75 strains were sequenced and analyzed. The KJ44 VP4 had 91.7% to 96.3% deduced amino acid identity to the bovine related P[1] strain, whereas the KJ75 strain was most closely related to the bovine related P[5] strain (91.9% to 96.9% amino acid identity). Both KJ44 and KJ75 strains also contained the bovine related VP3 gene. The remaining 9 segments were closely related to porcine group A rotaviruses. The KJ44 and KJ75 strains showed high amino acid identity to the G5 rotaviruses, sharing 90.4% to 99.0% identity. In addition, these strains belonged to the NSP4 genotype B, which is typical of porcine rotaviruses and subgroup I, with the closest relationship to the porcine JL-94 strain. These results strongly suggest that bovine rotavirus strains with the G5 genotype occur in nature as a novel G genotype in cattle as a result of a natural reassortment between bovine and porcine strains.

* Corresponding author. Mailing address: Veterinary Medical Research Center, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, South Korea. Phone: 82 62 530 2845. Fax: 82 62 530 2809. E-mail: choko{at}chonnam.ac.kr.

Journal of Clinical Microbiology, November 2006, p. 4101-4112, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.01196-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ha, T. P. M., Kim, H.-J., Saif, L. J., Jeong, Y.-J., Kim, H.-H., Kwon, H.-J., Park, S.-J., Cho, K.-O. (2009). Sequence Analysis of Unusual P[7]G5 Bovine Rotavirus Strains Reveals Evidence of Interspecies Transmission. J. Clin. Microbiol. 47: 3329-3332 [Abstract] [Full Text]  
  • Matthijnssens, J., Ciarlet, M., Heiman, E., Arijs, I., Delbeke, T., McDonald, S. M., Palombo, E. A., Iturriza-Gomara, M., Maes, P., Patton, J. T., Rahman, M., Van Ranst, M. (2008). Full Genome-Based Classification of Rotaviruses Reveals a Common Origin between Human Wa-Like and Porcine Rotavirus Strains and Human DS-1-Like and Bovine Rotavirus Strains. J. Virol. 82: 3204-3219 [Abstract] [Full Text]  


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