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Journal of Clinical Microbiology, November 2006, p. 4186-4188, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.01085-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Rapid Identification of Pathogens in Blood Cultures with a Modified Fluorescence In Situ Hybridization Assay

Remco P. H. Peters,1,2 Michiel A. van Agtmael,2 Alberdina M. Simoons-Smit,1 Sven A. Danner,2 Christina M. J. E. Vandenbroucke-Grauls,1,3 and Paul H. M. Savelkoul1*

Department of Medical Microbiology and Infection Control, VU University Medical Center Amsterdam, Amsterdam, The Netherlands,1 Department of Internal Medicine, VU University Medical Center, Amsterdam, The Netherlands,2 Department of Medical Microbiology, Academic Medical Center, Amsterdam, The Netherlands3

Received 25 May 2006/ Returned for modification 26 June 2006/ Accepted 25 July 2006

We evaluated a modified fluorescence in situ hybridization (FISH) assay for rapid (<1 h) identification of microorganisms in growth-positive blood cultures. The results were compared to those of the standard FISH technique and conventional culturing. The rapid identification of microorganisms with modified FISH can have important effects on clinical management of patients with bloodstream infections.

* Corresponding author. Mailing address: Department of Medical Microbiology and Infection Control, VU University Medical Center, P.O. Box 7057, 1007 MB Amsterdam, The Netherlands. Phone: 31 20 4440552. Fax: 31 20 4440473. E-mail: p.savelkoul{at}vumc.nl.

Journal of Clinical Microbiology, November 2006, p. 4186-4188, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.01085-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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  • Wellinghausen, N., Bartel, M., Essig, A., Poppert, S. (2007). Rapid Identification of Clinically Relevant Enterococcus Species by Fluorescence In Situ Hybridization. J. Clin. Microbiol. 45: 3424-3426 [Abstract] [Full Text]  


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