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Journal of Clinical Microbiology, November 2006, p. 4218-4221, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.01216-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Molecular Analysis of Clinical Isolates of Mycobacterium bovis Recovered from Humans in Italy{triangledown}

Nicoletta Lari,1 Laura Rindi,1 Daniela Bonanni,1 Enrico Tortoli,2 and Carlo Garzelli1*

Dipartimento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia ed Epidemiologia, Università di Pisa, I-56127 Pisa,1 Centro Regionale di Riferimento per i Micobatteri, Laboratorio di Microbiologia e Virologia, Ospedale Careggi, I-50134 Firenze, Italy2

Received 14 June 2006/ Returned for modification 29 August 2006/ Accepted 9 September 2006

In order to achieve a better knowledge of Mycobacterium bovis epidemiology in Italy, 42 clinical isolates from humans were genotyped. Predominant molecular patterns were found in one cluster of 15 isolates sharing spoligotype (ST482), variable-number tandem repeat (VNTR), and IS6110-based restriction fragment length polymorphism (one 1.9-kb band) profiles and in two clusters of 6 and 3 Mycobacterium bovis BCG isolates differing by one VNTR character. The remaining 18 isolates yielded unique profiles. Our results confirm the potential utility of spoligotyping and VNTR typing as a major typing system of M. bovis isolates.

* Corresponding author. Mailing address: Dipartimento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia ed Epidemiologia, Via San Zeno, 35/39 - I-56127 Pisa, Italy. Phone: 39 050 2213670. Fax: 39 050 2213671. E-mail: garzelli{at}biomed.unipi.it.

{triangledown} Published ahead of print on 20 September 2006.

Journal of Clinical Microbiology, November 2006, p. 4218-4221, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.01216-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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