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Journal of Clinical Microbiology, December 2006, p. 4376-4383, Vol. 44, No. 12
0095-1137/06/$08.00+0     doi:10.1128/JCM.01389-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Development of a Serotype-Specific DNA Microarray for Identification of Some Shigella and Pathogenic Escherichia coli Strains{triangledown}

Yayue Li,1,2,3,{dagger} Dan Liu,4,{dagger} Boyang Cao,1,2,3 Weiqing Han,1,2,3 Yanqun Liu,1,2,3 Fenxia Liu,1,2,3 Xi Guo,1,2,3 David A. Bastin,4 Lu Feng,1,2,3 and Lei Wang1,2,3,4*

TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 Hong Da Street, TEDA, Tianjin 300457, China,1 Tianjin Research Center for Functional Genomics and Biochips, TEDA College, Nankai University, Tianjin 300457, China,2 Tianjin Key Laboratory of Microbial Functional Genomics, TEDA College, Nankai University, 23 Hong Da Street, TEDA, Tianjin 300457, China,3 Tianjin Biochip Corporation, 23 HongDa Street, TEDA, Tianjin 300457, China4

Received 6 July 2006/ Returned for modification 10 August 2006/ Accepted 26 September 2006

Shigella and pathogenic Escherichia coli are major causes of human infectious diseases and are responsible for millions of cases of diarrhea worldwide every year. A convenient and rapid method to identify highly pathogenic serotypes of Shigella and E. coli is needed for large-scale epidemiologic study, timely clinical diagnosis, and reliable quarantine of the pathogens. In this study, a DNA microarray targeting O-serotype-specific genes was developed to detect 15 serotypes of Shigella and E. coli, including Shigella sonnei; Shigella flexneri type 2a; Shigella boydii types 7, 9, 13, 16, and 18; Shigella dysenteriae types 4, 8, and 10; and E. coli O55, O111, O114, O128, and O157. The microarray was tested against 186 representative strains of all Shigella and E. coli O serotypes, 38 clinical isolates, and 9 strains of other bacterial species that are commonly present in stool samples and was shown to be specific and reproducible. The detection sensitivity was 50 ng genomic DNA or 104 CFU per ml in mock stool specimens. This is the first report of a microarray for serotyping Shigella and pathogenic E. coli. The method has a number of advantages over traditional bacterial culture and antiserum agglutination methods and is promising for applications in basic microbiological research, clinical diagnosis, food safety, and epidemiological surveillance.

* Corresponding author. Mailing address: TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 Hong Da Street, TEDA, Tianjin 300457, China. Phone: 86-22-66229588. Fax: 86-22-66229596. E-mail: wanglei{at}nankai.edu.cn.

{triangledown} Published ahead of print on 4 October 2006.

{dagger} Y. Li and D. Liu contributed equally to this report.

Journal of Clinical Microbiology, December 2006, p. 4376-4383, Vol. 44, No. 12
0095-1137/06/$08.00+0     doi:10.1128/JCM.01389-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Li, Y., Cao, B., Liu, B., Liu, D., Gao, Q., Peng, X., Wu, J., Bastin, D. A., Feng, L., Wang, L. (2009). Molecular detection of all 34 distinct O-antigen forms of Shigella. J Med Microbiol 58: 69-81 [Abstract] [Full Text]  
  • Han, W., Liu, B., Cao, B., Beutin, L., Kruger, U., Liu, H., Li, Y., Liu, Y., Feng, L., Wang, L. (2007). DNA Microarray-Based Identification of Serogroups and Virulence Gene Patterns of Escherichia coli Isolates Associated with Porcine Postweaning Diarrhea and Edema Disease. Appl. Environ. Microbiol. 73: 4082-4088 [Abstract] [Full Text]  


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