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Journal of Clinical Microbiology, December 2006, p. 4541-4544, Vol. 44, No. 12
0095-1137/06/$08.00+0     doi:10.1128/JCM.01141-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Rapid Simultaneous Detection by Real-Time PCR of Cytomegalovirus UL97 Mutations in Codons 460 and 520 Conferring Ganciclovir Resistance{triangledown}

Katharina Göhring, Elfriede Mikeler, Gerhard Jahn, and Klaus Hamprecht*

Institute of Medical Virology and Epidemiology of Viral Diseases, University Hospital of Tübingen, 72076 Tübingen, Germany

Received 2 June 2006/ Returned for modification 29 August 2006/ Accepted 30 September 2006

Ganciclovir (GCV) resistance is an emerging problem for transplant recipients. A sensitive and rapid real-time PCR approach for simultaneous and semiquantitative detection of human cytomegalovirus (HCMV) UL97 mutations in codons 460/520 was established by LightCycler and confirmed by restriction fragment length polymorphism and sequencing. Results from HCMV laboratory strains were compared with results from 11 GCV-resistant clinical isolates.

* Corresponding author. Mailing address: Institute of Medical Virology and Epidemiology of Viral Diseases, University Hospital of Tübingen, Elfriede-Aulhorn-Str. 6, D-72076 Tübingen, Germany. Phone: 049-7071-2984657. Fax: 049-7071-295790. E-mail: kshampre{at}med.uni-tuebingen.de.

{triangledown} Published ahead of print on 11 October 2006.

Journal of Clinical Microbiology, December 2006, p. 4541-4544, Vol. 44, No. 12
0095-1137/06/$08.00+0     doi:10.1128/JCM.01141-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gohring, K., Feuchtinger, T., Mikeler, E., Lang, P., Jahn, G., Handgretinger, R., Hamprecht, K. (2009). Dynamics of the Emergence of a Human Cytomegalovirus UL97 Mutant Strain Conferring Ganciclovir Resistance in a Pediatric Stem-Cell Transplant Recipient. J. Mol. Diagn. 11: 364-368 [Abstract] [Full Text]  


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