This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jado, I.
Right arrow Articles by Anda, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jado, I.
Right arrow Articles by Anda, P.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, December 2006, p. 4572-4576, Vol. 44, No. 12
0095-1137/06/$08.00+0     doi:10.1128/JCM.01227-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Molecular Method for Identification of Rickettsia Species in Clinical and Environmental Samples{triangledown}

Isabel Jado,1,2 Raquel Escudero,1 Horacio Gil,1 María Isabel Jiménez-Alonso,1,{dagger} Rita Sousa,3 Ana L. García-Pérez,4 Manuela Rodríguez-Vargas,1 Bruno Lobo,1 and Pedro Anda1*

Laboratorio de Espiroquetas y Patógenos Especiales, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain,1 Unidad de Alertas y Emergencias, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain,2 Centro de Estudos de Vectores e Doenças Infecciosas (CEVDI), Instituto Nacional de Saude, Lisboa, Portugal,3 Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER), Derio, Vizcaya, Spain4

Received 15 June 2006/ Returned for modification 25 July 2006/ Accepted 22 September 2006

We present a PCR method targeting the 23S-5S internal transcribed spacer coupled with reverse line blotting that allows Rickettsia species detection and identification in a single step. The method is highly sensitive and specific in identifying Rickettsia species from both patient and environmental samples. The generic approach used allowed us to identify new pathogens.

* Corresponding author. Mailing address: Laboratorio de Espiroquetas y Patógenos Especiales, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220-Majadahonda, Madrid, Spain. Phone: 34 91 822 3601. Fax: 34 91 509 7966. E-mail: panda{at}isciii.es.

{triangledown} Published ahead of print on 11 October 2006.

{dagger} Present address: Servicio de Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.

Journal of Clinical Microbiology, December 2006, p. 4572-4576, Vol. 44, No. 12
0095-1137/06/$08.00+0     doi:10.1128/JCM.01227-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Toledo, A., Olmeda, A. S., Escudero, R., Jado, I., Valcarcel, F., Casado-Nistal, M. A., Rodriguez-Vargas, M., Gil, H., Anda, P. (2009). Tick-Borne Zoonotic Bacteria in Ticks Collected from Central Spain. Am J Trop Med Hyg 81: 67-74 [Abstract] [Full Text]  
  • Escudero, R., Toledo, A., Gil, H., Kovacsova, K., Rodriguez-Vargas, M., Jado, I., Garcia-Amil, C., Lobo, B., Bhide, M., Anda, P. (2008). Molecular Method for Discrimination between Francisella tularensis and Francisella-Like Endosymbionts. J. Clin. Microbiol. 46: 3139-3143 [Abstract] [Full Text]  
  • Garcia-Esteban, C., Gil, H., Rodriguez-Vargas, M., Gerrikagoitia, X., Barandika, J., Escudero, R., Jado, I., Garcia-Amil, C., Barral, M., Garcia-Perez, A. L., Bhide, M., Anda, P. (2008). Molecular Method for Bartonella Species Identification in Clinical and Environmental Samples. J. Clin. Microbiol. 46: 776-779 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»