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Journal of Clinical Microbiology, December 2006, p. 4577-4583, Vol. 44, No. 12
0095-1137/06/$08.00+0     doi:10.1128/JCM.01295-06

Prevalence and Sequence Variants of IS481 in Bordetella bronchiseptica: Implications for IS481-Based Detection of Bordetella pertussis ^,

Karen B. Register^1* and Gary N. Sanden²

Respiratory Diseases of Livestock Research Unit, USDA/Agricultural Research Service/National Animal Disease Center, P.O. Box 70, Ames, Iowa 50010,¹ U.S. Public Health Service Commissioned Corps, Centers for Disease Control and Prevention, National Center for Infectious Diseases, Division of Bacterial and Mycotic Disease, Meningitis and Special Pathogens Branch, Epidemiologic Investigations Laboratory, Atlanta, Georgia 30333²

Received 23 June 2006/ Returned for modification 10 August 2006/ Accepted 11 October 2006

We report the prevalence in Bordetella bronchiseptica of IS481, a frequent target for diagnosis of Bordetella pertussis, as approximately 5%. However, PCR amplicons of the predicted size were detectable in 78% of IS481-negative strains. Our results suggest that PCR targeting IS481 may not be sufficiently specific for reliable identification of B. pertussis.

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* Corresponding author. Mailing address: Respiratory Diseases of Livestock Research Unit, USDA/ARS/National Animal Disease Center, P.O. Box 70, 2300 Dayton Road, Ames, IA 50010. Phone: (515) 663-7700. Fax: (515) 663-7458. E-mail: kregiste{at}nadc.ars.usda.gov.

Published ahead of print on 25 October 2006.

Supplemental material for this article may be found at http://jcm.asm.org/.

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Journal of Clinical Microbiology, December 2006, p. 4577-4583, Vol. 44, No. 12
0095-1137/06/$08.00+0     doi:10.1128/JCM.01295-06

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