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Journal of Clinical Microbiology, February 2006, p. 580-582, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.580-582.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Two New Colorimetric Methods for Early Detection of Vancomycin and Oxacillin Resistance in Staphylococcus aureus

Ahmet Y. Coban,1 Bülent Bozdogan,2 Cigdem Cekic Cihan,1 Ebru Cetinkaya,1 Kemal Bilgin,1 Ozge Darka,1 Alper Akgunes,1 Belma Durupinar,1 and Peter C. Appelbaum3*

Ondokuz Mayis University, Medical School, Department of Microbiology and Clinical Microbiology, 55139 Samsun, Turkey,1 Adnan Menderes Universite Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji AD, 09100 Aydin, Turkey,2 The Penn State Milton S. Hershey Medical Center, Hershey, Pennsylvania3

Received 15 October 2005/ Returned for modification 28 November 2005/ Accepted 30 November 2005

We developed two colorimetric methods for the detection of vancomycin- and oxacillin-resistant Staphylococcus aureus in ≤6 h: (i) a nitrate reductase assay and (ii) a resazurin microplate method. MICs agreed with results obtained by CLSI methods for oxacillin. However, detection of vancomycin resistance required a larger inoculum. These methods may be recommended for the detection of vancomycin- and oxacillin-resistant S. aureus.

* Corresponding author. Mailing address: Department of Pathology, Hershey Medical Center, P.O. Box 850, Hershey, PA 17033. Phone: (717) 531-5113. Fax: (717) 531-7953. E-mail: pappelbaum{at}psu.edu.

Journal of Clinical Microbiology, February 2006, p. 580-582, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.580-582.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Acuner, I. C., Eroglu, C., Yilmaz Coban, A., Bozdogan, B., Durupinar, B., Appelbaum, P. C. (2006). Unacceptable Performance and the Lack of Reproducibility Results in the Report of Colorimetric Methods for Early Detection of Vancomycin and Oxacillin Resistance in Staphylococcus aureus.. J. Clin. Microbiol. 44: 2318-2319 [Full Text]  


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