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Journal of Clinical Microbiology, February 2006, p. 607-611, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.607-611.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Use of a New Dual-Antigen Enzyme-Linked Immunosorbent Assay To Detect and Characterize the Human Antibody Response to the Human Immunodeficiency Virus Type 2 Envelope gp125 and gp36 Glycoproteins

José Maria Marcelino,1 Helena Barroso,2,3 Fátima Gonçalves,4 Sofia Marques Silva,1 Carlos Novo,1 Perpétua Gomes,2,4 Ricardo Camacho,4 and Nuno Taveira2,3*

UTPAM, Departamento de Biotecnologia, Instituto Nacional de Engenharia Tecnologia e Inovação, Lisboa, Portugal,1 Instituto Superior de Ciências da Saúde Egas Moniz, Monte de Caparica, Portugal,2 Unidade de Retrovírus e Infecções Associadas, Centro de Patogénese Molecular, Faculdade de Farmácia de Lisboa, Lisboa, Portugal,3 Laboratório de Virologia, Serviço de Imuno-Hemoterapia, Hospital Egas Moniz, Lisboa, Portugal4

Received 7 July 2005/ Returned for modification 7 September 2005/ Accepted 21 November 2005

A dual-antigen enzyme-linked immunosorbent assay specific for human immunodeficiency virus type 2 (HIV-2) envelope proteins, ELISA-HIV2, was developed with two new recombinant polypeptides, rpC2-C3 and rgp36, derived from the HIV-2 envelope. The diagnostic performance was determined with HIV-2, HIV-1, and HIV-1/2 samples. Both polypeptides showed 100% specificity. Clinical sensitivity was 100% for rgp36 and 93.4% for rpC2-C3. ELISA-HIV2 may be used for the specific diagnosis and confirmation of HIV-2 infection.

* Corresponding author. Mailing address: Unidade de Retrovírus e Infecções Associadas, Centro de Patogénese Molecular, Faculdade de Farmácia de Lisboa, Avenida das Forças Armadas, 1649-019 Lisboa, Portugal. Phone: 351217934212. Fax: 351217934212. E-mail: ntaveira{at}mail.telepac.pt.

Journal of Clinical Microbiology, February 2006, p. 607-611, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.607-611.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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