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Journal of Clinical Microbiology, February 2006, p. 614-616, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.614-616.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Unique NS5b Hepatitis C Virus Gene Sequence Consensus Database Is Essential for Standardization of Genotype Determinations in Multicenter Epidemiological Studies

Syria Laperche,1 Karine Saune,2 Paul Dény,3 Gilles Duverlie,4 Sophie Alain,5 Marie-Laure Chaix,6 Catherine Gaudy,7 Françoise Lunel,8 Jean-Michel Pawlotsky,9 Christopher Payan,8 Bruno Pozzetto,10 Catherine Tamalet,11 Vincent Thibault,12 Sophie Vallet,13 Françoise Bouchardeau,1 Jacques Izopet,2 and Jean-Jacques Lefrère14,15*

Centre National de Référence pour les hépatites B et C en transfusion, Département des Agents Transmissibles par le Sang, Institut National de la Transfusion Sanguine, Paris, France,1 Laboratoire de Virologie, Centre Hospitalo-Universitaire Purpan, Toulouse, France,2 Laboratoire de Virologie-Bactériologie associé au CNR des hépatites B et C, Centre Hospitalo-Universitaire Avicenne, Bobigny, France,3 Laboratoire de Virologie, Centre Hospitalo-Universitaire, Amiens, France,4 Laboratoire de Virologie-Bactériologie, Centre Hospitalo-Universitaire, Limoges, France,5 Laboratoire de Virologie, Centre Hospitalo-Universitaire Necker, Paris, France,6 Université François Rabelais, EA 3856 et laboratoire de Bactériologie-Virologie, Centre Hospitalo-Universitaire Bretonneau, Tours, France,7 Laboratoire de Virologie-Bactériologie, Centre Hospitalo-Universitaire, Angers, France,8 Laboratoire de Virologie-Bactériologie, Centre Hospitalo-Universitaire Henri-Mondor, Créteil, France,9 Laboratoire de Virologie-Bactériologie, Centre Hospitalo-Universitaire, Saint Etienne, France,10 Laboratoire de Virologie- Bactériologie, Centre Hospitalo-Universitaire La Timone, Marseille, France,11 Laboratoire de Virologie, Centre Hospitalo-Universitaire Pitié-Salpêtrière, Paris, France,12 Laboratoire de Microbiologie, Centre Hospitalo-Universitaire, Brest, France,13 Laboratoire d'Hématologie, Centre Hospitalo-Universitaire, Amiens, France,14 Département des Agents Transmissibles par le Sang, Institut National de la Transfusion Sanguine, Paris, France,15

Received 13 September 2005/ Returned for modification 1 November 2005/ Accepted 29 November 2005

A multicenter study of NS5b hepatitis C virus (HCV) genotype determination involving 12 laboratories demonstrates that any laboratory with expertise in sequencing techniques would be able to provide a reliable HCV genotype for clinical and epidemiological purposes as long as they are provided a consensus reference sequence database.

* Corresponding author. Mailing address: Institut National de la Transfusion Sanguine, 6 rue Alexandre-Cabanel, 75015 Paris, France. Phone: 01 44 49 30 51. Fax: 01 44 49 30 59. E-mail: lefrere{at}ints.fr.

Journal of Clinical Microbiology, February 2006, p. 614-616, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.614-616.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Laperche, S., Bouchardeau, F., Thibault, V., Pozzetto, B., Vallet, S., Rosenberg, A. R., Roque-Afonso, A.-M., Gassin, M., Stoll-Keller, F., Trimoulet, P., Gault, E., Chanzy, B., Mercier, B., Branger, M., Pawlotsky, J.-M., Henquell, C., Lunel, F., Gaudy-Graffin, C., Alain, S., Chaix, M.-L., Duverlie, G., Izopet, J., Lefrere, J.-J. (2007). Multicenter Trials Need To Use the Same Assay for Hepatitis C Virus Viral Load Determination. J. Clin. Microbiol. 45: 3788-3790 [Abstract] [Full Text]  


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