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Journal of Clinical Microbiology, February 2006, p. 617-618, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.617-618.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Use of Commercially Available Cryogenic Vials for Long-Term Preservation of Dermatophyte Fungi

M. Baker1 and P. Jeffries2*

East Kent Microbiology Service, The William Harvey Hospital, Kennington Road, Ashford, Kent TN24 0LZ,1 Department of Biosciences, University of Kent, Canterbury, Kent CT2 6NJ, United Kingdom2

Received 26 August 2005/ Returned for modification 18 October 2005/ Accepted 23 November 2005

The use of commercially available cryogenic vials (Microbank vials) stored at –70°C for the storage and preservation of dermatophyte fungi was investigated. None of the 200 strains of dermatophytes examined, representing 21 species, showed a loss of viability after they had been stored for periods ranging from 1 week to 2 years at –70°C. All strains showed typical colonial and microscopic morphologies following revival.

* Corresponding author. Mailing address: East Kent Microbiology Service, The William Harvey Hospital, Kennington Road, Ashford, Kent TN24 0LZ, United Kingdom. Phone: 00441227823478. Fax: 00441227763912. E-mail: p.jeffries{at}kent.ac.uk.

Journal of Clinical Microbiology, February 2006, p. 617-618, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.617-618.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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