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Journal of Clinical Microbiology, March 2006, p. 1127-1131, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.1127-1131.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Consecutive Use of Two Multiplex PCR-Based Assays for Simultaneous Identification and Determination of Capsular Status of Nine Common Neisseria meningitidis Serogroups Associated with Invasive Disease

Désirée E. Bennett1 and Mary T. Cafferkey1,2*

Epidemiology and Molecular Biology Unit, The Children's University Hospital, Dublin,1 Department of Clinical Microbiology, Royal College of Surgeons in Ireland, York Street, Dublin, Ireland2

Received 2 June 2005/ Returned for modification 17 July 2005/ Accepted 23 December 2005

We developed two Neisseria meningitidis multiplex PCR assays to be used consecutively that allow determination of the serogroup and capsular status of serogroup A, B, C, 29E, W135, X, and Y cnl-3/cnl-1-like-containing N. meningitidis isolates by direct analysis of the amplicon size. These assays offer a rapid and simple method of serogrouping N. meningitidis.

* Corresponding author. Mailing address: Epidemiology and Molecular Biology Unit, The Children's University Hospital, Temple Street, Dublin 1, Ireland. Phone: 353-1-878-4858. Fax: 353-1-878-4856. E-mail: M.Cafferkey{at}tsch.ie.

Journal of Clinical Microbiology, March 2006, p. 1127-1131, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.1127-1131.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Fraisier, C., Stor, R., Tenebray, B., Sanson, Y., Nicolas, P. (2009). Use of a New Single Multiplex PCR-Based Assay for Direct Simultaneous Characterization of Six Neisseria meningitidis Serogroups. J. Clin. Microbiol. 47: 2662-2666 [Abstract] [Full Text]  


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