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Journal of Clinical Microbiology, March 2006, p. 1141-1144, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.1141-1144.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Novel Multiplex PCR Assay for Detection of the Staphylococcal Virulence Marker Panton-Valentine Leukocidin Genes and Simultaneous Discrimination of Methicillin-Susceptible from -Resistant Staphylococci

Jo-Ann McClure,1 John M. Conly,1,2,3,4,5 Vicky Lau,1 Sameer Elsayed,2,3,5 Thomas Louie,3,4 Wendy Hutchins,3 and Kunyan Zhang1,2,3,4,5*

Centre for Antimicrobial Resistance, Calgary Health Region/Calgary Laboratory Services/University of Calgary, Calgary, Alberta, Canada,1 Departments of Pathology & Laboratory Medicine,2 Microbiology and Infectious Diseases,3 Medicine, University of Calgary, Calgary, Alberta, Canada,4 Calgary Laboratory Services, Calgary, Alberta, Canada5

Received 3 October 2005/ Returned for modification 28 November 2005/ Accepted 4 January 2006

We developed a new multiplex PCR assay for detection of Panton-Valentine leukocidin virulence genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci. This assay is simple, rapid, and accurate and offers the potential for prompt detection of newly emerging community-associated methicillin-resistant Staphylococcus aureus.

* Corresponding author. Mailing address: Department of Pathology & Laboratory Medicine, University of Calgary, #9-3535 Research Road N.W., Calgary, Alberta T2L 2K8, Canada. Phone: (403) 770-3583. Fax: (403) 770-3347. E-mail: kunyan.zhang{at}cls.ab.ca.

Journal of Clinical Microbiology, March 2006, p. 1141-1144, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.1141-1144.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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