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Journal of Clinical Microbiology, March 2006, p. 1197-1201, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.1197-1201.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

CASE REPORT

Use of Enzyme-Linked Immunospot Assay with Mycobacterium tuberculosis- Specific Peptides for Diagnosis of Recent Infection with M. tuberculosis after Accidental Laboratory Exposure

Eliane M. S. Leyten,1* Bert Mulder,3 Corine Prins,1 Karin Weldingh,4 Peter Andersen,4 Tom H. M. Ottenhoff,2 Jaap T. van Dissel,1 and Sandra M. Arend1*

Department of Infectious Diseases,1 Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands,2 Laboratory of Medical Microbiology Twente Achterhoek, Enschede, The Netherlands,3 Department of Infectious Disease Immunology, Statens Serum Institute, Copenhagen, Denmark4

Received 1 December 2005/ Accepted 27 December 2005

This report of an accidental exposure to Mycobacterium tuberculosis in a microbiological laboratory illustrates the value of gamma interferon enzyme-linked immunospot assay using peptides of ESAT-6, CFP-10, TB37.6, and TB7.7 for the diagnosis of latent infection. In particular, positive responses to peptides 2 to 6 of TB37.6 were observed exclusively in recently infected persons.

* Corresponding author. Mailing address: Dept. of Infectious Diseases, C5P, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands. Phone: 31 71 526 26 13. Fax: 31 71 526 67 58. E-mail for Sandra M. Arend: S.M.Arend{at}LUMC.nl. E-mail for Elaine M. S. Leyten: e.m.s.leyten{at}LUMC.nl.

Journal of Clinical Microbiology, March 2006, p. 1197-1201, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.1197-1201.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cehovin, A., Cliff, J. M., Hill, P. C., Brookes, R. H., Dockrell, H. M. (2007). Extended Culture Enhances Sensitivity of a Gamma Interferon Assay for Latent Mycobacterium tuberculosis Infection. CVI 14: 796-798 [Abstract] [Full Text]  
  • Pai, M., Joshi, R., Dogra, S., Mendiratta, D. K., Narang, P., Kalantri, S., Reingold, A. L., Colford, J. M. Jr., Riley, L. W., Menzies, D. (2006). Serial Testing of Health Care Workers for Tuberculosis Using Interferon-{gamma} Assay. Am. J. Respir. Crit. Care Med. 174: 349-355 [Abstract] [Full Text]  


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