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Journal of Clinical Microbiology, April 2006, p. 1447-1452, Vol. 44, No. 4
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.4.1447-1452.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Use of a Serotype-Specific DNA Microarray for Identification of Group B Streptococcus (Streptococcus agalactiae)

Linyan Wen,1,2 Quan Wang,1,2 Yayue Li,1,2 Fanrong Kong,3 Gwendolyn L. Gilbert,3 Boyang Cao,1,2 Lei Wang,1,2 and Lu Feng1,2*

TEDA School of Biological Sciences and Biotechnology, Nankai University, 23# HongDa Street, TEDA, Tianjin 300457, People's Republic of China,1 Tianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, 23# HongDa Street, TEDA, Tianjin 300457, People's Republic of China,2 Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, 2145 New South Wales, Australia3

Received 20 October 2005/ Returned for modification 3 January 2006/ Accepted 25 January 2006

Group B Streptococcus (GBS; Streptococcus agalactiae) is an important cause of sepsis and meningitis. Nine GBS serotypes, based on capsular polysaccharide (CPS) antigens, have been described. Their distribution varies worldwide and needs to be monitored to understand the epidemiology of GBS disease and inform the development of vaccines. In this study, we sequenced cpsH of GBS serotype II (cpsHII) and compared it with that of the other eight serotypes to identify serotype-specific regions. We then developed a DNA microarray based on the cpsH gene and used it to test 88 GBS isolates—9 serotype reference strains and 79 clinical isolates—and 7 other bacterial and fungal species which are commonly present in the vagina flora. The microarray was shown to be specific and reproducible. This is the first report of a microarray which can identify the nine GBS serotypes. The use of a microarray has advantages over traditional serotyping methods and will be of practical value in both reference and diagnostic laboratories.

* Corresponding author. Mailing address: TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 HongDa Street, TEDA, Tianjin 300457, People's Republic of China. Phone: 86-22-66229592. Fax: 86-22-66229596. E-mail: fenglu63{at}nankai.edu.cn.

Journal of Clinical Microbiology, April 2006, p. 1447-1452, Vol. 44, No. 4
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.4.1447-1452.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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