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Journal of Clinical Microbiology, April 2006, p. 1571-1574, Vol. 44, No. 4
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.4.1571-1574.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Human Herpesvirus 6 Chromosomal Integration in Immunocompetent Patients Results in High Levels of Viral DNA in Blood, Sera, and Hair Follicles

Katherine N. Ward,1* Hoe Nam Leong,2 Elisabeth P. Nacheva,3 Julie Howard,3 Claire E. Atkinson,2 Nicholas W. S. Davies,4 Paul D. Griffiths,2 and Duncan A. Clark2,{dagger}

Division of Infection and Immunity, Centre for Virology, Windeyer Institute of Medical Sciences, Royal Free and University College Medical School (University College London C Campus), 46 Cleveland Street, London W1T 4JF,1 Division of Infection and Immunity, Centre for Virology,2 Department of Haematology, Royal Free and University College Medical School (Hampstead campus), Rowland Hill Street, London NW3 2PF,3 Department of Neurological Intensive Care, The National Hospital for Neurology and Neurosurgery, London WC1N 3BG, United Kingdom4

Received 9 February 2006/ Accepted 13 February 2006

Six immunocompetent patients with human herpesvirus 6 (HHV-6) chromosomal integration had HHV-6 and ß-globin DNA quantified in various samples by PCR. The mean HHV-6 DNA concentration (log10 copies/milliliter) in blood was 7.0 (≥1 HHV-6 DNA copies/leukocyte), and in serum it was 5.3 (≥1 HHV-6 DNA copies/lysed cell). The mean HHV-6 DNA load (log10 copies)/hair follicle was 4.2 (≥1 copies/hair follicle cell), demonstrating that viral integration is not confined to blood cells. The characteristically high HHV-6 DNA levels in chromosomal integration may confound laboratory diagnosis of HHV-6 infection and should be given due consideration.

* Corresponding author. Mailing address: Centre for Virology, Division of Infection and Immunity, Royal Free and University College Medical School (UCL campus), Windeyer Institute of Medical Sciences, 46 Cleveland St., London W1T 4JF, United Kingdom. Phone: 44 (0)20-7679-9134. Fax: 44 (0)20-7580-5896. E-mail: k.n.ward{at}ucl.ac.uk.

{dagger} Present address: Virology, Barts & the London NHS Trust, London E1 2ES, United Kingdom.

Journal of Clinical Microbiology, April 2006, p. 1571-1574, Vol. 44, No. 4
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.4.1571-1574.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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